Neurotensin stimulates the sperm acrosome reaction and reduces percentages of fertilization in vitro.
Contraception
fertility
in vitro fertilization
neurotensin
sperm acrosome reaction
Journal
F&S science
ISSN: 2666-335X
Titre abrégé: F S Sci
Pays: United States
ID NLM: 101765857
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
28
01
2020
revised:
02
03
2020
accepted:
13
03
2020
entrez:
13
5
2022
pubmed:
1
8
2020
medline:
1
8
2020
Statut:
ppublish
Résumé
To determine the impact of neurotensin (NTS), a naturally occurring peptide, on the function of human and nonhuman primate sperm. Experimental study. University-based research laboratory. Consenting normozoospermic human donors and cynomolgus macaques. None. Sperm acrosome status was assessed. Computer-assisted semen analysis assessed sperm motility, progression, and velocity. Immunocytochemistry and receptor selective agonists were used to identify specific NTS receptors on sperm. Monkey oocytes were obtained after ovarian stimulation, and NTS-treated monkey sperm were used for in vitro fertilization. Neurotensin treatment of human sperm stimulated the acrosome reaction in both a dose-dependent (0.1-10 μmol/L) and time-dependent (5-30 minutes) manner. Neurotensin treatment did not alter sperm motility or progression. Both a general NTS receptor antagonist (SR142948) and a NTSR1 selective antagonist (SR48692) reduced the ability of NTS to stimulate the acrosome reaction. The neurotensin receptor NTSR1, but not NTSR2 or SORT1, was detected in monkey sperm using immunostaining. Neurotensin treatment also compromised the ability of sperm to fertilize an oocyte. Percentage of fertilization with untreated monkey sperm and monkey oocytes was 72%. Sperm pre-treated with NTS yielded a significantly lower fertilization rate of 18%. Neurotensin effectively stimulates the acrosome reaction in human and monkey sperm. Neurotensin produced by the oviduct or cumulus cells may promote natural fertilization. Pretreatment of sperm with NTS significantly reduces fertilization. Exposure of sperm to NTS prior to reaching the oviduct has the potential for contraceptive development. Identification of NTSR1 as the mediator of NTS action provides a specific target for future studies.
Identifiants
pubmed: 35559737
pii: S2666-335X(20)30001-X
doi: 10.1016/j.xfss.2020.03.001
pmc: PMC10034862
mid: NIHMS1878036
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
27-35Subventions
Organisme : NICHD NIH HHS
ID : P01 HD071875
Pays : United States
Informations de copyright
Copyright © 2020 American Society for Reproductive Medicine. All rights reserved.
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