A fertilin-derived peptide improves in vitro maturation and ploidy of human oocytes.

Aneuploidy Comparative Genomic Hybridization Fertilins / metabolism Humans Oocytes Peptides / metabolism Ploidies
FLNA FMN1 Human oocyte chromosome segregation invitro maturation

Journal

F&S science
ISSN: 2666-335X
Titre abrégé: F S Sci
Pays: United States
ID NLM: 101765857

Informations de publication

Date de publication:
02 2022
Historique:
received: 13 09 2021
revised: 19 10 2021
accepted: 29 10 2021
entrez: 13 5 2022
pubmed: 14 5 2022
medline: 18 5 2022
Statut: ppublish

Résumé

To analyze the effect of a cyclic fertilin-derived peptide (cFEE) on in vitro maturation of human oocytes. Randomized study. Fertility center in an academic hospital. Not applicable. Human immature germinal vesicle-stage oocytes (n = 1,629) donated for research according to French bioethics laws were randomly allocated to groups treated with 1 or 100 μM of cFEE or to a control group. They were incubated at 37 °C in 6% CO The percentage of oocytes undergoing maturation in vitro was observed. Aneuploidy and euploidy were assessed for all chromosomes, and differential gene expression was analyzed in oocytes treated with cFEE compared with the control to obtain insights into its mechanism of action. cFEE significantly increased the percentage of oocytes that matured in vitro and improved euploidy in meiosis II oocytes by the up-regulation of FMN1 and FLNA genes, both of which encode proteins involved in spindle structure. cFEE improves human oocyte maturation in vitro and reduces aneuploidy. It may prove useful for treating oocytes before fertilization in assisted reproductive technology and for in vitro maturation in fertility preservation programs to improve oocyte quality and the chances for infertile couples to conceive.

Identifiants

DOI: 10.1016/j.xfss.2021.10.004 PMID: 35559993
pubmed: 35559993
pii: S2666-335X(21)00084-7
doi: 10.1016/j.xfss.2021.10.004
pii:
doi:

Substances chimiques

Peptides 0
Fertilins EC 3.4.24.-

Types de publication

Journal Article Randomized Controlled Trial

Langues

eng

Sous-ensembles de citation

IM

Pagination

21-28

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Amira Sallem (A)

Team "From Gametes to Birth," Département Développement, Reproduction, Cancer, Institut Cochin, Institut de la Santé et de la Recherche Médicale U1016, Centre National de la Recherche Scientifique UMR8104, Université de Paris, 22 rue Mechain, Paris, France; Laboratoire d'Histologie-Embryologie et Cytogénétique, Faculté de Médecine de Monastir, Tunisie.

Anne-Lyse Denizot (AL)

Team "From Gametes to Birth," Département Développement, Reproduction, Cancer, Institut Cochin, Institut de la Santé et de la Recherche Médicale U1016, Centre National de la Recherche Scientifique UMR8104, Université de Paris, 22 rue Mechain, Paris, France; Service d'Histologie-Embryologie-Biologie de la Reproduction, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Paris, France.

Ahmed Ziyyat (A)

Team "From Gametes to Birth," Département Développement, Reproduction, Cancer, Institut Cochin, Institut de la Santé et de la Recherche Médicale U1016, Centre National de la Recherche Scientifique UMR8104, Université de Paris, 22 rue Mechain, Paris, France; Service d'Histologie-Embryologie-Biologie de la Reproduction, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Paris, France.

Audrey L'Hostis (A)

Team "From Gametes to Birth," Département Développement, Reproduction, Cancer, Institut Cochin, Institut de la Santé et de la Recherche Médicale U1016, Centre National de la Recherche Scientifique UMR8104, Université de Paris, 22 rue Mechain, Paris, France.

Sophie Favier (S)

Team "From Gametes to Birth," Département Développement, Reproduction, Cancer, Institut Cochin, Institut de la Santé et de la Recherche Médicale U1016, Centre National de la Recherche Scientifique UMR8104, Université de Paris, 22 rue Mechain, Paris, France.

Philippe Burlet (P)

Service de Génétique Moléculaire, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France.

Jean-Michel Lapierre (JM)

Service d'Histologie-Embryologie-Cytogénétique, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France.

Solohaja Faniaha Dimby (SF)

Unité de Recherche Clinique, ACTION Study Group, Hôpital Fernand Widal, Assistance Publique-Hôpitaux de Paris, Paris, France; Statistique, Analyse et Modélisation Multidisciplinaire-EA 4543, Université Paris 1 Panthéon Sorbonne, Paris, France.

Catherine Patrat (C)

Team "From Gametes to Birth," Département Développement, Reproduction, Cancer, Institut Cochin, Institut de la Santé et de la Recherche Médicale U1016, Centre National de la Recherche Scientifique UMR8104, Université de Paris, 22 rue Mechain, Paris, France; Service d'Histologie-Embryologie-Biologie de la Reproduction, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Paris, France.

Christophe Sifer (C)

Service d'Histologie-Embryologie-Cytogénétique-Centre d'Etude et de Conservation des Œufs et du Sperme humains, Centre Hospitalo-Universitaire Jean Verdier, Assistance Publique-Hôpitaux de Paris, Bondy, France.

Eric Vicaut (E)

Unité de Recherche Clinique, ACTION Study Group, Hôpital Fernand Widal, Assistance Publique-Hôpitaux de Paris, Paris, France.

Julie Steffann (J)

Service de Génétique Moléculaire, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France; Institut Imagine, Université de Paris, Laboratoire des Maladies Génétiques Mitochondriales. Inserm 1163, Paris, France.

Daniel Vaiman (D)

Team "From Gametes to Birth," Département Développement, Reproduction, Cancer, Institut Cochin, Institut de la Santé et de la Recherche Médicale U1016, Centre National de la Recherche Scientifique UMR8104, Université de Paris, 22 rue Mechain, Paris, France.

Serge Pierrick Romana (SP)

Service d'Histologie-Embryologie-Cytogénétique, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France; Institut Imagine, Université de Paris, Laboratoire d'Embryologie et de Génétique des Malformations Congénitales, Institut de la Santé et de la Recherche Médicale 1163, Paris, France.

Jean-Philippe Wolf (JP)

Team "From Gametes to Birth," Département Développement, Reproduction, Cancer, Institut Cochin, Institut de la Santé et de la Recherche Médicale U1016, Centre National de la Recherche Scientifique UMR8104, Université de Paris, 22 rue Mechain, Paris, France; Service d'Histologie-Embryologie-Biologie de la Reproduction, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Paris, France. Electronic address: jean-philippe.wolf@aphp.fr.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Ploïdies › Aneuploïdie › A fertilin-derived peptide improves in vitro...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Hybridation génomique comparative › A fertilin-derived peptide improves in vitro...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Glycoprotéines › Fertilines › A fertilin-derived peptide improves in vitro...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A fertilin-derived peptide improves in vitro...
questionsmedicales.fr › Cellules › Cellules germinales › Ovocytes › A fertilin-derived peptide improves in vitro...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › A fertilin-derived peptide improves in vitro...
questionsmedicales.fr › Phénomènes génétiques › Ploïdies › A fertilin-derived peptide improves in vitro...