Unique expression patterns of the embryonal stem cell marker SOX2 and hormone receptors suggest the existence of a subpopulation of epithelial stem/progenitor cells in porcine and bovine endometrium.
SOX2
endometrium
epithelial stem/progenitor cells
farm animals
hormone receptors
Journal
Veterinary medicine and science
ISSN: 2053-1095
Titre abrégé: Vet Med Sci
Pays: England
ID NLM: 101678837
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
pubmed:
14
5
2022
medline:
23
7
2022
entrez:
13
5
2022
Statut:
ppublish
Résumé
There are currently insufficient data on the population of endometrial epithelial stem/progenitor cells in farm animals. With the aim of identifying a potential population of epithelial stem/progenitor cells in the porcine and bovine endometrium, this study immunohistochemically examined the expression patterns of the oestrogen and progesterone receptors, as well as that of the embryonal stem cell marker SOX2. A total of 24 endometrial tissue samples obtained from cycling pigs (n = 12) and cows (n = 12) were included in our study. Each endometrium was divided into basal, middle and luminal portions. The percentage of marker-positive cells and the intensity of the immunoreaction in each portion of the endometrium were determined. Inverse expression patterns of SOX2 and progesterone receptors were found in both animal species throughout the oestrous cycle. Strong diffuse SOX2 expression was detected in the basal portions of the glands, while a significant decrease in positivity and a weak immunoreaction were found in the luminal two thirds of the glandular epithelium. Strong progesterone receptor expression was observed in at least 90% of glandular cells in the middle and luminal portions, whereas weak staining and significant decrease in positivity were detected in the basal portions of the glands. One oestrogen receptor expression pattern resembled that of progesterone receptors. The inverse expression patterns of SOX2 and hormone (especially progesterone) receptors suggest that endometrial epithelial stem/progenitor cells represent a subset of cells that reside in the basal portions of the endometrial glands in both the bovine and porcine endometrium.
Sections du résumé
BACKGROUND
There are currently insufficient data on the population of endometrial epithelial stem/progenitor cells in farm animals.
OBJECTIVES
With the aim of identifying a potential population of epithelial stem/progenitor cells in the porcine and bovine endometrium, this study immunohistochemically examined the expression patterns of the oestrogen and progesterone receptors, as well as that of the embryonal stem cell marker SOX2.
METHODS
A total of 24 endometrial tissue samples obtained from cycling pigs (n = 12) and cows (n = 12) were included in our study. Each endometrium was divided into basal, middle and luminal portions. The percentage of marker-positive cells and the intensity of the immunoreaction in each portion of the endometrium were determined.
RESULTS
Inverse expression patterns of SOX2 and progesterone receptors were found in both animal species throughout the oestrous cycle. Strong diffuse SOX2 expression was detected in the basal portions of the glands, while a significant decrease in positivity and a weak immunoreaction were found in the luminal two thirds of the glandular epithelium. Strong progesterone receptor expression was observed in at least 90% of glandular cells in the middle and luminal portions, whereas weak staining and significant decrease in positivity were detected in the basal portions of the glands. One oestrogen receptor expression pattern resembled that of progesterone receptors.
CONCLUSION
The inverse expression patterns of SOX2 and hormone (especially progesterone) receptors suggest that endometrial epithelial stem/progenitor cells represent a subset of cells that reside in the basal portions of the endometrial glands in both the bovine and porcine endometrium.
Identifiants
pubmed: 35561288
doi: 10.1002/vms3.802
pmc: PMC9297784
doi:
Substances chimiques
Biomarkers
0
Receptors, Estrogen
0
Receptors, Progesterone
0
Progesterone
4G7DS2Q64Y
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1489-1501Informations de copyright
© 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
Références
Reproduction. 2003 Mar;125(3):301-11
pubmed: 12611594
Nat Commun. 2020 Apr 14;11(1):1805
pubmed: 32286318
Mol Cell Endocrinol. 2008 Jun 25;288(1-2):22-9
pubmed: 18403104
Int J Mol Sci. 2015 May 14;16(5):10934-51
pubmed: 26006231
Endocr Rev. 2006 Apr;27(2):208-19
pubmed: 16434509
Dev Biol. 2013 Feb 15;374(2):281-94
pubmed: 23274420
Iran Biomed J. 2016;20(2):109-16
pubmed: 26568058
Clin Lab. 2014;60(6):909-18
pubmed: 25016694
Reprod Domest Anim. 2017 Oct;52(5):707-714
pubmed: 28419571
Mol Cell Biol. 1989 Apr;9(4):1415-25
pubmed: 2786140
Anim Reprod Sci. 2011 Apr;124(3-4):251-8
pubmed: 21397415
Hum Reprod Update. 2016 Mar-Apr;22(2):137-63
pubmed: 26552890
Adv Biochem Eng Biotechnol. 2009;114:185-99
pubmed: 19343304
Annu Rev Cell Dev Biol. 2009;25:377-406
pubmed: 19575646
Ann N Y Acad Sci. 1991;622:47-56
pubmed: 2064204
Reprod Domest Anim. 2014 Aug;49(4):550-559
pubmed: 24754629
Aust N Z J Obstet Gynaecol. 2004 Oct;44(5):380-6
pubmed: 15387855
Reprod Biol Endocrinol. 2003 Jun 21;1:50
pubmed: 12848893
Reproduction. 2015 Apr;149(4):317-27
pubmed: 25556182
Clin Cancer Res. 2007 Aug 1;13(15 Pt 1):4415-21
pubmed: 17671124
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021 Mar;165(1):69-79
pubmed: 32158015
J Biosci Bioeng. 2005 Jul;100(1):12-27
pubmed: 16233846
Vet Med Sci. 2022 Jul;8(4):1489-1501
pubmed: 35561288
Curr Top Dev Biol. 2005;68:85-122
pubmed: 16124997
Hum Reprod. 2013 Oct;28(10):2695-708
pubmed: 23847113
Am J Obstet Gynecol. 1976 Mar 15;124(6):582-95
pubmed: 943943
J Dairy Sci. 1980 Jan;63(1):155-60
pubmed: 7372895
Fertil Steril. 2005 Oct;84 Suppl 2:1124-30
pubmed: 16210003
Cell Syst. 2020 Jun 24;10(6):480-494.e8
pubmed: 32553182
PLoS One. 2010 Apr 28;5(4):e10387
pubmed: 20442847