The Golgi Method.

Neurons Silver Staining / methods Staining and Labeling

Journal

Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530

Informations de publication

Date de publication:
01 08 2022
Historique:
pubmed: 14 5 2022
medline: 4 8 2022
entrez: 13 5 2022
Statut: epublish

Résumé

Silver staining by the Golgi method is a classical procedure to identify the three-dimensional morphology of individual neurons. Although the method was developed in the 1870s, it is still used to study the morphological characteristics of neuron types in the central nervous system, either alone or in combination with other neuroanatomical techniques. The neuropil of insects is fully accessible to the original refractive staining procedure, and modifications of the Golgi technique have paved the path for modern structural research on the insect central nervous system. Here, we provide an introduction to this easy and low-cost method.

Identifiants

DOI: 10.1101/pdb.top107695 PMID: 35562111
pubmed: 35562111
pii: pdb.top107695
doi: 10.1101/pdb.top107695
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

Pdb.top107695

Informations de copyright

© 2022 Cold Spring Harbor Laboratory Press.

Auteurs

Rickard Ignell (R)

Disease Vector Group, Unit of Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, 23422 Alnarp, Sweden rickard.ignell@slu.se.

Sharon Rose Hill (SR)

Disease Vector Group, Unit of Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, 23422 Alnarp, Sweden.

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Classifications MeSH

questionsmedicales.fr › Cellules › Neurones › The Golgi Method.
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › Coloration à l'argent › The Golgi Method.
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › The Golgi Method.