Anti-Inflammatory and Anti-Fibrotic Effect of Immortalized Mesenchymal-Stem-Cell-Derived Conditioned Medium on Human Lung Myofibroblasts and Epithelial Cells.
A549
ADSCs
conditioned medium
fibrosis
idiopathic pulmonary fibrosis
inflammation
mesenchymal stem cells
pulmonary subepithelial myofibroblasts
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
20 Apr 2022
20 Apr 2022
Historique:
received:
30
03
2022
revised:
18
04
2022
accepted:
19
04
2022
entrez:
14
5
2022
pubmed:
15
5
2022
medline:
18
5
2022
Statut:
epublish
Résumé
Idiopathic pulmonary fibrosis (IPF) is caused by progressive lung tissue impairment due to extended chronic fibrosis, and it has no known effective treatment. The use of conditioned media (CM) from an immortalized human adipose mesenchymal stem cell line could be a promising therapeutic strategy, as it can reduce both fibrotic and inflammatory responses. We aimed to investigate the anti-inflammatory and anti-fibrotic effect of CM on human pulmonary subepithelial myofibroblasts (hPSM) and on A549 pulmonary epithelial cells, treated with pro-inflammatory or pro-fibrotic mediators. CM inhibited the proinflammatory cytokine-induced mRNA and protein production of various chemokines in both hPSMs and A549 cells. It also downregulated the mRNA expression of IL-1α, but upregulated IL-1β and IL-6 mRNA production in both cell types. CM downregulated the pro-fibrotic-induced mRNA expression of collagen Type III and the migration rate of hPSMs, but upregulated fibronectin mRNA production and the total protein collagen secretion. CM's direct effect on the chemotaxis and cell recruitment of immune-associated cells, and its indirect effect on fibrosis through the significant decrease in the migration capacity of hPSMs, makes it a plausible candidate for further development towards a therapeutic treatment for IPF.
Identifiants
pubmed: 35562961
pii: ijms23094570
doi: 10.3390/ijms23094570
pmc: PMC9102072
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Culture Media, Conditioned
0
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Int J Mol Sci. 2019 May 05;20(9):
pubmed: 31060326
BMC Pulm Med. 2021 Dec 7;21(1):404
pubmed: 34876074
Cytokine. 2012 Jun;58(3):415-23
pubmed: 22484241
Stem Cell Res Ther. 2020 Sep 15;11(1):399
pubmed: 32933584
Toxicol Lett. 2020 Mar 15;321:103-113
pubmed: 31706003
Chest. 2011 Nov;140(5):1243-1253
pubmed: 21835903
N Engl J Med. 2015 Mar 26;372(13):1189-91
pubmed: 25806913
Expert Rev Respir Med. 2019 Jan;13(1):39-51
pubmed: 30526140
Sci Transl Med. 2019 Sep 25;11(511):
pubmed: 31554736
Inflammation. 2020 Feb;43(1):123-134
pubmed: 31646446
Life Sci. 2021 Dec 15;287:120123
pubmed: 34748761
Diagnostics (Basel). 2020 Jul 03;10(7):
pubmed: 32635390
Stem Cell Res Ther. 2018 Jan 12;9(1):7
pubmed: 29329595
Chest. 2016 Sep;150(3):533-43
pubmed: 27020420
Eur J Immunol. 1999 Feb;29(2):530-6
pubmed: 10064068
Front Med (Lausanne). 2018 May 15;5:142
pubmed: 29868594
Stem Cells Int. 2021 Jul 27;2021:9929461
pubmed: 34354753
Antioxid Redox Signal. 2013 Jan 1;18(1):66-79
pubmed: 22703534
Am J Transl Res. 2020 Sep 15;12(9):4998-5014
pubmed: 33042402
Nat Commun. 2019 Jul 5;10(1):2987
pubmed: 31278260
Biochemistry (Mosc). 2019 Nov;84(11):1375-1389
pubmed: 31760924
Am J Respir Cell Mol Biol. 2020 Nov;63(5):623-636
pubmed: 32730709
Stem Cell Res Ther. 2018 Dec 4;9(1):335
pubmed: 30514375
Front Immunol. 2016 Nov 11;7:483
pubmed: 27891127
J Immunol Methods. 2002 Jan 1;259(1-2):55-64
pubmed: 11730841
Lab Anim Res. 2018 Mar;34(1):1-10
pubmed: 29628971
Clin Respir J. 2022 Feb;16(2):84-96
pubmed: 35001525
Am J Physiol Lung Cell Mol Physiol. 2012 Dec 1;303(11):L967-77
pubmed: 23023971
Cytokine. 2021 Oct;146:155643
pubmed: 34332275
Ecotoxicol Environ Saf. 2021 Apr 10;216:112181
pubmed: 33848736
PLoS Negl Trop Dis. 2016 Apr 26;10(4):e0004669
pubmed: 27115869
Sci Rep. 2021 Mar 11;11(1):5762
pubmed: 33707643
Am J Physiol Lung Cell Mol Physiol. 2018 Nov 1;315(5):L697-L710
pubmed: 30091381
Nat Rev Dis Primers. 2017 Oct 20;3:17074
pubmed: 29052582
BMC Pulm Med. 2017 Oct 20;17(1):133
pubmed: 29058630
Front Immunol. 2013 Jul 15;4:182
pubmed: 23874332
Chest. 2016 Mar;149(3):756-66
pubmed: 26836914
Anal Cell Pathol (Amst). 2021 Aug 13;2021:6653681
pubmed: 34426780
Cytotherapy. 2014 Jan;16(1):17-32
pubmed: 24094500
Phytomedicine. 2020 Nov;78:153298
pubmed: 32781391
Medicina (Kaunas). 2019 Mar 16;55(3):
pubmed: 30884853
Int J Mol Med. 2018 Feb;41(2):659-668
pubmed: 29207055
Front Pharmacol. 2021 Jul 28;12:669037
pubmed: 34393771
Stem Cell Res Ther. 2021 May 20;12(1):295
pubmed: 34016178
Life Sci. 2020 Feb 15;243:117290
pubmed: 31923420
Chest. 2017 May;151(5):971-981
pubmed: 27890713
Am J Physiol Lung Cell Mol Physiol. 2019 Dec 1;317(6):L823-L831
pubmed: 31553626
J Extracell Vesicles. 2021 Aug;10(10):e12124
pubmed: 34377373
Int Immunopharmacol. 2019 May;70:208-215
pubmed: 30851700
Expert Opin Biol Ther. 2016;16(3):375-87
pubmed: 26593230
J Natl Cancer Inst. 1973 Nov;51(5):1417-23
pubmed: 4357758
Am J Respir Crit Care Med. 2021 Jul 15;204(2):197-208
pubmed: 33689671
Eur Rev Med Pharmacol Sci. 2017 Sep;21(18):4007-4013
pubmed: 29028103
Am J Manag Care. 2017 Jul;23(11 Suppl):S176-S182
pubmed: 28978212
Eur Respir Rev. 2019 Oct 1;28(153):
pubmed: 31578210
Mol Immunol. 2017 Oct;90:11-21
pubmed: 28662409
Eur Respir Rev. 2019 Sep 4;28(153):
pubmed: 31484664
J Med Invest. 2001 Aug;48(3-4):181-9
pubmed: 11694958
Eur Respir J. 2005 Apr;25(4):701-7
pubmed: 15802346
Respir Res. 2019 Oct 30;20(1):239
pubmed: 31666086
Expert Opin Biol Ther. 2020 Feb;20(2):125-140
pubmed: 31701782
Expert Rev Respir Med. 2020 Apr;14(4):405-414
pubmed: 31994940
Respir Res. 2018 Nov 9;19(1):218
pubmed: 30413158
Pulm Pharmacol Ther. 2017 Oct;46:78-87
pubmed: 28865842
J Allergy Clin Immunol. 2003 Jun;111(6):1185-99; quiz 1200
pubmed: 12789214
Stem Cell Res Ther. 2021 Sep 16;12(1):506
pubmed: 34530920
J Cell Sci. 2020 Jul 10;133(13):
pubmed: 32651236
Front Genet. 2021 May 12;12:644143
pubmed: 34054916
Immunity. 2021 Sep 14;54(9):2042-2056.e8
pubmed: 34407391
Eur Respir J. 2021 Oct 14;58(4):
pubmed: 33795318
BMC Pulm Med. 2018 Apr 27;18(1):63
pubmed: 29703175
Biomedicines. 2021 Oct 05;9(10):
pubmed: 34680516
Forensic Sci Med Pathol. 2018 Jun;14(2):174-179
pubmed: 29607464
Am J Respir Cell Mol Biol. 2021 Jan;64(1):115-125
pubmed: 33074715
Stem Cells Transl Med. 2020 Sep;9(9):1023-1035
pubmed: 32452646
J Cell Mol Med. 2021 Jun 2;:
pubmed: 34076355
Am J Med Sci. 2019 May;357(5):390-393
pubmed: 31010466
Histol Histopathol. 2020 Mar;35(3):289-301
pubmed: 31318036
J Clin Invest. 1999 Oct;104(8):1061-9
pubmed: 10525044
PLoS Pathog. 2012;8(7):e1002833
pubmed: 22911498
Am J Respir Crit Care Med. 2014 Oct 15;190(8):867-78
pubmed: 25090037
Int J Mol Sci. 2018 Sep 30;19(10):
pubmed: 30274394
PLoS One. 2014 Dec 31;9(12):e116142
pubmed: 25551570
Stem Cell Res Ther. 2021 Feb 3;12(1):96
pubmed: 33536061
Inflamm Bowel Dis. 2018 Sep 15;24(10):2224-2241
pubmed: 29860326
J Manag Care Spec Pharm. 2017 Mar;23(3-b Suppl):S5-S16
pubmed: 28287346
Expert Rev Respir Med. 2021 Feb;15(2):239-247
pubmed: 32981357
Arch Iran Med. 2020 Dec 01;23(12):870-879
pubmed: 33356346
Int J Radiat Oncol Biol Phys. 2018 Jul 15;101(4):945-956
pubmed: 29976507
Br J Pharmacol. 2016 Oct;173(19):2859-79
pubmed: 27448286
J Immunother Cancer. 2019 Feb 11;7(1):42
pubmed: 30744691
J Oral Sci. 2016;58(1):101-8
pubmed: 27021546
Cytokine. 2005 Sep 7;31(5):375-81
pubmed: 16051495