Cell Model of Depression: Reduction of Cell Stress with Mirtazapine.
SH-SY5Y cells
comet assay
depression
hydrogen peroxide
mirtazapine
stress
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
29 Apr 2022
29 Apr 2022
Historique:
received:
08
02
2022
revised:
26
04
2022
accepted:
27
04
2022
entrez:
14
5
2022
pubmed:
15
5
2022
medline:
18
5
2022
Statut:
epublish
Résumé
Depression is a very prevalent and complex disease. This condition is associated with a high rate of relapse, making its treatment a challenge. Thus, an intensive investigation of this disease and its treatment is necessary. In this work, through cell viability assays (MTT and neutral red assays) and alkaline comet assays, we aimed to test the induction of stress in human SH-SY5Y cells through the application of hydrocortisone and hydrogen peroxide and to test the reversal or attenuation of this stress through the application of mirtazapine to the cells. Our results demonstrated that hydrogen peroxide, and not hydrocortisone, can induce cellular stress, as evidenced by DNA damage and a global cellular viability reduction, which were alleviated by the antidepressant mirtazapine. The establishment of a cellular model of depression through stress induction is important to study new possibilities of treatment of this disease using cell cultures.
Identifiants
pubmed: 35563333
pii: ijms23094942
doi: 10.3390/ijms23094942
pmc: PMC9099543
pii:
doi:
Substances chimiques
Mirtazapine
A051Q2099Q
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Neural Plast. 2016;2016:8056370
pubmed: 26881133
Neurotoxicol Teratol. 2018 Sep - Oct;69:27-38
pubmed: 30076895
Nanomaterials (Basel). 2021 Nov 27;11(12):
pubmed: 34947574
Nat Rev Dis Primers. 2016 Sep 15;2:16065
pubmed: 27629598
Front Cell Neurosci. 2020 Nov 11;14:554613
pubmed: 33262689
Int J Immunopathol Pharmacol. 2018 Jan-Dec;32:2058738418785739
pubmed: 29983082
Neurochem Res. 2009 Apr;34(4):746-54
pubmed: 18802751
Psychiatry Investig. 2016 Nov;13(6):616-621
pubmed: 27909452
J Food Biochem. 2021 Feb;45(2):e13592
pubmed: 33349958
Pharmacol Res Perspect. 2019 May 03;7(3):e00472
pubmed: 31065377
Drug Discov Today. 2020 Jul;25(7):1270-1276
pubmed: 32404275
Med Sci Monit. 2015 Feb 06;21:412-8
pubmed: 25656523
Nat Rev Endocrinol. 2019 Sep;15(9):525-534
pubmed: 31249398
Pharmaceuticals (Basel). 2021 Sep 16;14(9):
pubmed: 34577630
Sci Rep. 2016 Jul 22;6:30187
pubmed: 27443987
Antioxidants (Basel). 2019 Dec 26;9(1):
pubmed: 31888114
Front Mol Neurosci. 2021 Jun 03;14:661728
pubmed: 34149353
BMC Complement Altern Med. 2014 Oct 08;14:384
pubmed: 25293674
Int J Mol Sci. 2019 May 31;20(11):
pubmed: 31151322
NMR Biomed. 2019 Mar;32(3):e4057
pubmed: 30707463
J Clin Diagn Res. 2014 Dec;8(12):CC04-7
pubmed: 25653939
Pharmacol Rep. 2020 Jun;72(3):563-570
pubmed: 32240535
Front Neurosci. 2019 Mar 08;13:188
pubmed: 30906247
Molecules. 2020 Jun 18;25(12):
pubmed: 32570881