MicroRNA-449a Inhibits Triple Negative Breast Cancer by Disturbing DNA Repair and Chromatid Separation.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
04 May 2022
Historique:
received: 31 03 2022
revised: 26 04 2022
accepted: 03 05 2022
entrez: 14 5 2022
pubmed: 15 5 2022
medline: 18 5 2022
Statut: epublish

Résumé

Chromosomal instability (CIN) can be a driver of tumorigenesis but is also a promising therapeutic target for cancer associated with poor prognosis such as triple negative breast cancer (TNBC). The treatment of TNBC cells with defects in DNA repair genes with poly(ADP-ribose) polymerase inhibitor (PARPi) massively increases CIN, resulting in apoptosis. Here, we identified a previously unknown role of microRNA-449a in CIN. The transfection of TNBC cell lines HCC38, HCC1937 and HCC1395 with microRNA-449a mimics led to induced apoptosis, reduced cell proliferation, and reduced expression of genes in homology directed repair (HDR) in microarray analyses.

Identifiants

pubmed: 35563522
pii: ijms23095131
doi: 10.3390/ijms23095131
pmc: PMC9102308
pii:
doi:

Substances chimiques

Antineoplastic Agents 0
MicroRNAs 0
Poly(ADP-ribose) Polymerase Inhibitors 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Beate Vajen (B)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Rahul Bhowmick (R)

Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.

Luisa Greiwe (L)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Vera Schäffer (V)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Marlies Eilers (M)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Thea Reinkens (T)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Amelie Stalke (A)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Gunnar Schmidt (G)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Jan Fiedler (J)

Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.
Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Nikolai-Fuchs Str. 1, 30625 Hannover, Germany.

Thomas Thum (T)

Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.
Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Nikolai-Fuchs Str. 1, 30625 Hannover, Germany.
REBIRTH Center for Translational Regenerative Medicine, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

David S DeLuca (DS)

German Center for Lung Research (BREATH), Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Ian D Hickson (ID)

Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.

Brigitte Schlegelberger (B)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Thomas Illig (T)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

Britta Skawran (B)

Department of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30629 Hannover, Germany.

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Classifications MeSH