Ovocystatin Induced Changes in Expression of Alzheimer's Disease Relevant Proteins in APP/PS1 Transgenic Mice.
Alzheimer’s disease
chicken cystatin
cystatin C
mice
ovocystatin
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
23 Apr 2022
23 Apr 2022
Historique:
received:
15
02
2022
revised:
08
04
2022
accepted:
19
04
2022
entrez:
14
5
2022
pubmed:
15
5
2022
medline:
15
5
2022
Statut:
epublish
Résumé
Background: Ovocystatin is marked by structural and biological similarities to human cystatin C, which plays an important role in the course of neurodegenerative diseases. Recently, it has been shown that ovocystatin might prevent aging-related cognitive impairment in rats and reduce memory decline in an APP/PS1 mice model. Thus, this study aimed to assess the effect of ovocystatin on histopathological changes in APP/PS1 mice. Materials and methods: Ovocystatin was administered intraperitoneally for four weeks (40 μg/mouse) to 35-weeks-old transgenic (AD, n = 14) and wild type (NCAR, n = 15) mice (stock B6C3-Tg(APPswe, PSEN1dE9)85Dbo/Mmjax). A histopathological evaluation comprised antibodies directed against β-amyloid (1:400, SIG-39320-1000, Covance) and Tau (1:4000, AHB0042, Invitrogen). Three regions of the hippocampus— the dentate gyrus (DG) and the cornu ammonis (CA1 and CA3)—were analyzed by immunohistochemistry in each animal. All differences are expressed as percentage relative to the control group. Results: The main results showed that the percentage of immunoreactive area of β-amyloid, tau protein deposits in APP/PS1+ovCYS was decreased in DG, CA1, and CA3 regions compared with the APP/PS1 control, respectively (p < 0.05). Conclusions: Ovocystatin caused significant changes in the expression pattern of all investigated proteins in hippocampal tissues both in APP/PS1 and NCAR mice.
Identifiants
pubmed: 35566501
pii: jcm11092372
doi: 10.3390/jcm11092372
pmc: PMC9103311
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Polish Ministry of Science and Higher Educations
ID : SUBZ.C230.22.062
Organisme : "Innovative Technologies of production of biopreparations based on new generations of eggs (OVOCURA)"
ID : POIG 01.03.01-00-133/08
Références
Int J Psychiatry Clin Pract. 2015 Mar;19(1):2-7
pubmed: 25195764
Neuron. 2008 Oct 23;60(2):247-57
pubmed: 18957217
Scand J Clin Lab Invest. 2007;67(2):179-90
pubmed: 17365997
J Cell Sci. 2007 Dec 1;120(Pt 23):4081-91
pubmed: 18032783
Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):3046-51
pubmed: 15705720
Neurobiol Aging. 2004 Sep;25(8):1033-43
pubmed: 15212828
Front Mol Neurosci. 2012 Jul 06;5:79
pubmed: 22783166
J Biol Chem. 2008 Mar 21;283(12):7745-53
pubmed: 18184658
Int J Mol Sci. 2019 Jan 20;20(2):
pubmed: 30669512
Fluids Barriers CNS. 2018 Jul 1;15(1):19
pubmed: 29960602
Adv Clin Exp Med. 2017 Jul;26(4):563-569
pubmed: 28691424
Int J Biol Macromol. 2017 Sep;102:674-693
pubmed: 28445699
PLoS One. 2010 Mar 23;5(3):e9819
pubmed: 20352108
J Neuropathol Exp Neurol. 2001 Jan;60(1):94-104
pubmed: 11202179
Eur J Neurol. 2018 Jan;25(1):59-70
pubmed: 28872215
J Alzheimers Dis. 2011;26(2):387-408
pubmed: 21613740
Ageing Res Rev. 2016 Dec;32:22-37
pubmed: 27125852
J Mol Biol. 1993 Dec 20;234(4):1060-9
pubmed: 8263913
Mater Sociomed. 2016 Jun;28(3):235-8
pubmed: 27482169
Nat Genet. 2007 Dec;39(12):1440-2
pubmed: 18026100
Expert Rev Neurother. 2008 May;8(5):687-9
pubmed: 18457524
Alzheimers Dement. 2016 Mar;12(3):292-323
pubmed: 27012484
Neurogenetics. 2005 May;6(2):85-9
pubmed: 15776278
Am J Pathol. 2001 Sep;159(3):1061-8
pubmed: 11549598
Cell Death Dis. 2014 Oct 30;5:e1497
pubmed: 25356866
Lancet Neurol. 2015 Apr;14(4):388-405
pubmed: 25792098
Neuropharmacology. 2015 Sep;96(Pt A):94-104
pubmed: 24978103
Ageing Res Rev. 2016 Dec;32:38-50
pubmed: 27333827
Sci Adv. 2019 Jan 23;5(1):eaau3333
pubmed: 30746447
Nat Rev Neurol. 2018 Jul;14(7):399-415
pubmed: 29895964
Alzheimers Dement. 2021 Mar;17(3):327-406
pubmed: 33756057
Biol Chem. 1998 Nov;379(11):1371-5
pubmed: 9865612
Brain Res. 2005 Dec 20;1066(1-2):120-8
pubmed: 16325785
Biomol Eng. 2001 Jun;17(6):157-65
pubmed: 11337275
Adv Med Sci. 2019 Mar;64(1):65-71
pubmed: 30504006
Psychiatr Danub. 2016 Sep;28(Suppl-1):136-140
pubmed: 27663824
Biomed Res Int. 2019 Jun 16;2019:9171424
pubmed: 31317043
Biol Chem. 1996 Dec;377(12):847-50
pubmed: 8997496
Nat Rev Dis Primers. 2015 Oct 15;1:15056
pubmed: 27188934
Curr Neuropharmacol. 2017;15(7):996-1009
pubmed: 28294067
Nat Genet. 2007 Dec;39(12):1437-9
pubmed: 18026102
Biochem Soc Symp. 2003;(70):179-99
pubmed: 14587292
Mol Cell Neurosci. 2018 Jun;89:1-8
pubmed: 29577984
J Biol Chem. 2013 Jun 28;288(26):18853-62
pubmed: 23671283
Curr Pharm Des. 2017;23(40):6206-6214
pubmed: 28914201
Crit Rev Oral Biol Med. 1992;3(4):307-32
pubmed: 1391414
J Diet Suppl. 2018 Nov 2;15(6):977-1009
pubmed: 29281341
Front Biosci (Schol Ed). 2011 Jan 01;3(2):541-54
pubmed: 21196395
Science. 2008 Feb 22;319(5866):1086-9
pubmed: 18202255
Science. 1997 Jan 31;275(5300):630-1
pubmed: 9019820
J Alzheimers Dis. 2010;19(3):885-94
pubmed: 20157244
Anal Biochem. 1972 May;47(1):280-93
pubmed: 4624156
Anal Biochem. 1987 Nov 1;166(2):368-79
pubmed: 2449095
Front Neurol. 2015 Sep 02;6:178
pubmed: 26388830
Hum Mol Genet. 2004 Jan 15;13(2):159-70
pubmed: 14645205
Int J Mol Sci. 2021 Jul 30;22(15):
pubmed: 34360973