Single-cell analysis implicates T
PPP
Single-cell RNA sequencing
T-cell plasticity
palmoplantar pustulosis
scRNA-Seq
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
20
10
2021
revised:
04
03
2022
accepted:
20
04
2022
pubmed:
15
5
2022
medline:
12
10
2022
entrez:
14
5
2022
Statut:
ppublish
Résumé
Palmoplantar pustulosis (PPP) is a severe inflammatory skin disorder characterized by eruptions of painful, neutrophil-filled pustules on the palms and soles. Although PPP has a profound effect on quality of life, it remains poorly understood and notoriously difficult to treat. We sought to investigate the immune pathways that underlie the pathogenesis of PPP. We applied bulk and single-cell RNA sequencing (RNA-Seq) methods to the analysis of skin biopsy samples and peripheral blood mononuclear cells. We validated our results by flow cytometry and immune fluorescence microscopy RESULTS: Bulk RNA-Seq of patient skin detected an unexpected signature of T-cell activation, with a significant overexpression of several T PPP is associated with complex T-cell activation patterns and may explain why biologic drugs that target individual T helper cell populations have shown limited therapeutic efficacy.
Sections du résumé
BACKGROUND
Palmoplantar pustulosis (PPP) is a severe inflammatory skin disorder characterized by eruptions of painful, neutrophil-filled pustules on the palms and soles. Although PPP has a profound effect on quality of life, it remains poorly understood and notoriously difficult to treat.
OBJECTIVE
We sought to investigate the immune pathways that underlie the pathogenesis of PPP.
METHODS
We applied bulk and single-cell RNA sequencing (RNA-Seq) methods to the analysis of skin biopsy samples and peripheral blood mononuclear cells. We validated our results by flow cytometry and immune fluorescence microscopy RESULTS: Bulk RNA-Seq of patient skin detected an unexpected signature of T-cell activation, with a significant overexpression of several T
CONCLUSIONS
PPP is associated with complex T-cell activation patterns and may explain why biologic drugs that target individual T helper cell populations have shown limited therapeutic efficacy.
Identifiants
pubmed: 35568077
pii: S0091-6749(22)00624-8
doi: 10.1016/j.jaci.2022.04.027
pii:
doi:
Substances chimiques
Biological Products
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
882-893Subventions
Organisme : Medical Research Council
ID : MR/R015643/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/T02383X/1
Pays : United Kingdom
Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.