Stress-induced increase of monoclonal antibody production in CHO cells.
CHO
cell size
cell‐specific productivity
mAb
stress
Journal
Engineering in life sciences
ISSN: 1618-0240
Titre abrégé: Eng Life Sci
Pays: Germany
ID NLM: 101193313
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
received:
01
06
2021
revised:
07
02
2022
accepted:
11
02
2022
entrez:
16
5
2022
pubmed:
17
5
2022
medline:
17
5
2022
Statut:
epublish
Résumé
Monoclonal antibodies (mAbs) are of great interest to the biopharmaceutical industry due to their widely used application as human therapeutic and diagnostic agents. As such, mAb require to exhibit human-like glycolization patterns. Therefore, recombinant Chinese hamster ovary (CHO) cells are the favored production organisms; many relevant biopharmaceuticals are already produced by this cell type. To optimize the mAb yield in CHO DG44 cells a corelation between stress-induced cell size expansion and increased specific productivity was investigated. CO
Identifiants
pubmed: 35573136
doi: 10.1002/elsc.202100062
pii: ELSC1482
pmc: PMC9077828
doi:
Types de publication
Journal Article
Langues
eng
Pagination
427-436Informations de copyright
© 2022 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH.
Déclaration de conflit d'intérêts
We confirm that all corresponding authors agree with the submission and publication of this paper and that there is no conflict of interest concerning financial and personal relationships. The manuscript does not contain neither experiments using animals nor human studies. Furthermore, we confirm that the article has not been published previously by any of the authors and is not under consideration for publication elsewhere at the time of submission.
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