Scalable fabrication, compartmentalization and applications of living microtissues.
Clinical translation
Industrial translation
Microfluidics
Micromaterials
Tissue engineering
Upscaling
Journal
Bioactive materials
ISSN: 2452-199X
Titre abrégé: Bioact Mater
Pays: China
ID NLM: 101685294
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
30
12
2021
revised:
18
03
2022
accepted:
06
04
2022
entrez:
16
5
2022
pubmed:
17
5
2022
medline:
17
5
2022
Statut:
epublish
Résumé
Living microtissues are used in a multitude of applications as they more closely resemble native tissue physiology, as compared to 2D cultures. Microtissues are typically composed of a combination of cells and materials in varying combinations, which are dictated by the applications' design requirements. Their applications range wide, from fundamental biological research such as differentiation studies to industrial applications such as cruelty-free meat production. However, their translation to industrial and clinical settings has been hindered due to the lack of scalability of microtissue production techniques. Continuous microfluidic processes provide an opportunity to overcome this limitation as they offer higher throughput production rates as compared to traditional batch techniques, while maintaining reproducible control over microtissue composition and size. In this review, we provide a comprehensive overview of the current approaches to engineer microtissues with a focus on the advantages of, and need for, the use of continuous processes to produce microtissues in large quantities. Finally, an outlook is provided that outlines the required developments to enable large-scale microtissue fabrication using continuous processes.
Identifiants
pubmed: 35574053
doi: 10.1016/j.bioactmat.2022.04.005
pii: S2452-199X(22)00174-8
pmc: PMC9062422
doi:
Types de publication
Journal Article
Langues
eng
Pagination
392-405Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare they have no conflict of interest.The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Science. 2005 Apr 22;308(5721):537-41
pubmed: 15845850
J Thorac Cardiovasc Surg. 2006 Oct;132(4):918-24
pubmed: 17000305
Trends Biotechnol. 2021 Nov;39(11):1144-1159
pubmed: 33602609
Bioact Mater. 2018 Feb 14;3(2):167-173
pubmed: 29744454
Nat Mater. 2015 Jul;14(7):737-44
pubmed: 26030305
J Chem Technol Biotechnol. 2017 Jul;92(7):1577-1589
pubmed: 28706339
Biomicrofluidics. 2012 Oct 24;6(4):44104
pubmed: 24155865
Macromol Biosci. 2012 Jul;12(7):946-51
pubmed: 22311460
Integr Biol (Camb). 2014 Sep;6(9):874-84
pubmed: 25036382
Angew Chem Int Ed Engl. 2010 Aug 9;49(34):5846-68
pubmed: 20572214
Adv Sci (Weinh). 2018 Oct 24;6(1):1801076
pubmed: 30643716
Regen Ther. 2017 Jul 17;7:24-33
pubmed: 30271849
Nat Methods. 2014 Aug;11(8):847-54
pubmed: 24973948
Eur Cell Mater. 2012 Jun 05;23:387-99
pubmed: 22665161
Adv Funct Mater. 2020 Oct 22;30(43):
pubmed: 33708028
Tissue Eng. 2005 Nov-Dec;11(11-12):1658-66
pubmed: 16411811
Biomed Mater. 2019 Apr 05;14(3):034104
pubmed: 30844766
Adv Mater. 2016 Jun;28(21):4032-9
pubmed: 27000493
Lab Chip. 2018 Aug 7;18(16):2466-2476
pubmed: 30033460
Langmuir. 2004 Nov 9;20(23):9905-8
pubmed: 15518471
Drug Discov Today. 2015 Feb;20(2):236-46
pubmed: 25448757
Nat Rev Cardiol. 2018 Nov;15(11):659-671
pubmed: 29743563
Small. 2017 Jun;13(22):
pubmed: 28452168
Glob Chall. 2020 Nov 04;5(2):2000056
pubmed: 33552551
Cardiovasc Res. 2006 Feb 1;69(2):466-75
pubmed: 16423569
Int J Radiat Biol Relat Stud Phys Chem Med. 1970;18(5):491-5
pubmed: 5316564
Soft Matter. 2007 Jul 17;3(8):986-992
pubmed: 32900048
Front Mol Biosci. 2020 Mar 06;7:33
pubmed: 32211418
Sci Rep. 2019 Apr 18;9(1):6291
pubmed: 31000733
Adv Funct Mater. 2020 Jun;30(26):1909009
pubmed: 35677899
Analyst. 2011 Feb 7;136(3):473-8
pubmed: 20967331
Biomaterials. 2011 Aug;32(24):5625-32
pubmed: 21601276
J Sci Food Agric. 2014 Apr;94(6):1039-41
pubmed: 24214798
Nat Methods. 2021 Apr;18(4):346-357
pubmed: 33828274
Adv Healthc Mater. 2017 Feb;6(3):
pubmed: 27973710
Adv Mater. 2021 Oct;33(42):e2102660
pubmed: 34476848
Diabetes. 2015 Aug;64(8):2723-34
pubmed: 25795216
Lab Chip. 2006 Mar;6(3):437-46
pubmed: 16511628
Biosci Biotechnol Biochem. 2019 Dec;83(12):2272-2275
pubmed: 31482750
Lab Chip. 2019 Jun 7;19(11):1977-1984
pubmed: 31017163
Biotechnol J. 2018 Feb;13(2):
pubmed: 29193872
ACS Appl Mater Interfaces. 2015 Jul 1;7(25):13925-33
pubmed: 26053733
Pharm Res. 1991 Mar;8(3):341-4
pubmed: 2052521
Nat Cell Biol. 2018 Aug;20(8):979-989
pubmed: 30038254
Science. 1980 Nov 21;210(4472):908-10
pubmed: 6776628
ACS Appl Mater Interfaces. 2016 Mar 9;8(9):5906-16
pubmed: 26886167
Lab Chip. 2012 Aug 21;12(16):2881-7
pubmed: 22688131
Sci Rep. 2017 Jun 29;7(1):4363
pubmed: 28663555
Mater Today Bio. 2020 Mar 06;6:100047
pubmed: 32300754
Nat Commun. 2018 Oct 3;9(1):4049
pubmed: 30282987
Sci Adv. 2019 Sep 06;5(9):eaaw2459
pubmed: 31523707
Adv Mater. 2017 Nov;29(43):
pubmed: 28991390
Lab Chip. 2013 Dec 7;13(23):4525-33
pubmed: 24113543
Biomed Microdevices. 2012 Apr;14(2):313-23
pubmed: 22057945
Methods Mol Biol. 2016;1422:33-40
pubmed: 27246020
J Microencapsul. 2008 Oct;25(7):443-68
pubmed: 19238722
Lab Chip. 2002 Feb;2(1):24-6
pubmed: 15100856
Acta Biomater. 2020 Sep 1;113:63-83
pubmed: 32561471
Nature. 2018 May;557(7703):106-111
pubmed: 29720634
Sci Rep. 2017 Nov 6;7(1):14610
pubmed: 29097768
Angew Chem Int Ed Engl. 2005 Jan 21;44(5):724-8
pubmed: 15612064
Biomaterials. 2013 Dec;34(36):9010-7
pubmed: 23978517
Stem Cells Int. 2019 Nov 7;2019:4607461
pubmed: 31814836
Biomaterials. 2013 Nov;34(33):8122-30
pubmed: 23927952
Macromol Biosci. 2016 Oct;16(10):1524-1532
pubmed: 27440382
Biomater Res. 2015 Nov 04;19:23
pubmed: 26543592
Biomaterials. 2020 Feb;231:119664
pubmed: 31855623
J Biomech Eng. 2021 Oct 1;143(10):
pubmed: 33973004
Biotechnol Bioeng. 2012 Jun;109(6):1561-70
pubmed: 22234803
World J Gastroenterol. 2012 Oct 7;18(37):5145-50
pubmed: 23066307
Electrophoresis. 2013 Feb;34(3):425-31
pubmed: 23161405
Sci Adv. 2018 Jan 31;4(1):eaao1175
pubmed: 29399628
Gels. 2017 May 30;3(2):
pubmed: 30920517
Proc Natl Acad Sci U S A. 2015 Feb 24;112(8):2527-32
pubmed: 25675525
Acta Biomater. 2019 Sep 1;95:3-31
pubmed: 30481608
Molecules. 2019 May 07;24(9):
pubmed: 31067670
Physiol Genomics. 2014 May 1;46(9):309-14
pubmed: 24619520
Science. 2006 Oct 13;314(5797):298-300
pubmed: 17038622
Biochim Biophys Acta. 2014 Aug;1840(8):2506-19
pubmed: 24418517
Nat Commun. 2014 Jun 30;5:4250
pubmed: 24977495
Adv Healthc Mater. 2015 Aug 5;4(11):1628-33
pubmed: 26039892
Assay Drug Dev Technol. 2014 May;12(4):207-18
pubmed: 24831787
Stem Cells. 2007 Sep;25(9):2224-34
pubmed: 17585171
Cell Commun Signal. 2011 Sep 13;9:18
pubmed: 21914164
3D Print Addit Manuf. 2020 Jun 1;7(3):139-147
pubmed: 32855996
Lab Chip. 2006 Aug;6(8):1073-9
pubmed: 16874381
Front Bioeng Biotechnol. 2020 Jul 16;8:767
pubmed: 32766220
Macromol Rapid Commun. 2010 Jan 18;31(2):108-18
pubmed: 21590882
Adv Mater. 2020 Jun;32(24):e1907966
pubmed: 32346909
Small. 2016 Apr 27;12(16):2130-45
pubmed: 27101419
Angew Chem Int Ed Engl. 2012 Oct 1;51(40):10109-13
pubmed: 22936631
Nat Mater. 2007 Feb;6(2):162-7
pubmed: 17237789
ACS Biomater Sci Eng. 2017 Aug 14;3(8):1519-1526
pubmed: 33429638
Front Bioeng Biotechnol. 2021 Jan 28;8:620962
pubmed: 33585419
Sci Rep. 2018 Jul 27;8(1):11314
pubmed: 30054522
Adv Mater. 2014 Apr 23;26(16):2592-9
pubmed: 24395427
Biomaterials. 2013 Jan;34(1):11-8
pubmed: 23069710
Regen Ther. 2018 Aug 24;9:45-57
pubmed: 30525075
Nat Commun. 2021 May 10;12(1):2581
pubmed: 33972544
Adv Mater. 2016 Apr;28(16):3202-8
pubmed: 26915480
Nat Commun. 2019 Sep 25;10(1):4347
pubmed: 31554812
Lab Chip. 2016 Apr 26;16(9):1593-604
pubmed: 27025278
Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):14843-8
pubmed: 23980147
Stem Cell Reports. 2021 May 11;16(5):1143-1155
pubmed: 33891872
EBioMedicine. 2021 Dec;74:103717
pubmed: 34839265
Micromachines (Basel). 2020 Jul 30;11(8):
pubmed: 32751579
Adv Mater. 2017 Aug;29(32):
pubmed: 28650574
Biotechnol Bioeng. 2003 Jul 20;83(2):173-80
pubmed: 12768623
Lab Chip. 2009 Feb 21;9(4):516-20
pubmed: 19190786
Tissue Eng Part A. 2018 Mar;24(5-6):418-431
pubmed: 28825364
Nat Mater. 2011 Sep 04;10(11):877-83
pubmed: 21892177
Soft Matter. 2005 May 27;1(1):23-27
pubmed: 32646073
IEEE Trans Biomed Circuits Syst. 2009 Aug;3(4):195-201
pubmed: 23853240
Biomed Mater. 2019 Sep 09;14(6):065001
pubmed: 31498778
ACS Biomater Sci Eng. 2016 Apr 11;2(4):697-706
pubmed: 33465869
Sci Rep. 2021 Oct 29;11(1):21296
pubmed: 34716334
ACS Appl Mater Interfaces. 2018 Jul 18;10(28):23433-23438
pubmed: 29952552
Small. 2019 Sep;15(39):e1903147
pubmed: 31410986
Biomicrofluidics. 2014 Feb 03;8(1):016502
pubmed: 24753732
Trends Biotechnol. 2018 Aug;36(8):850-865
pubmed: 29656795
Am J Transplant. 2019 May;19(5):1315-1327
pubmed: 30378751
Biomaterials. 2016 Sep;102:20-42
pubmed: 27318933
Nanomicro Lett. 2021 Aug 18;13(1):182
pubmed: 34409511
Comput Struct Biotechnol J. 2020 Jun 02;18:1320-1329
pubmed: 32612755
Acta Biomater. 2018 Sep 1;77:48-62
pubmed: 30006317
Transplant Proc. 2011 Nov;43(9):3188-91
pubmed: 22099753
Tissue Eng. 2001 Jun;7(3):247-65
pubmed: 11429146
Soft Matter. 2009;5(7):1312-1319
pubmed: 20179781
Biotechnol Prog. 2003 Mar-Apr;19(2):243-53
pubmed: 12675556
Lab Chip. 2011 Feb 21;11(4):620-4
pubmed: 21125099
Langmuir. 2006 Oct 24;22(22):9453-7
pubmed: 17042568
Sci Rep. 2020 Jun 11;10(1):9468
pubmed: 32528073
Drug Dev Ind Pharm. 2001 Apr;27(4):309-19
pubmed: 11411898
Sci Rep. 2016 Nov 03;6:36011
pubmed: 27808102
Biomed Microdevices. 2009 Aug;11(4):739-46
pubmed: 19242806
Adv Sci (Weinh). 2020 Jul 19;7(18):2001419
pubmed: 32999847
Acta Biomater. 2021 Feb;121:204-213
pubmed: 33271356
Front Chem. 2019 Apr 25;7:258
pubmed: 31106194
RSC Adv. 2020 Jan 2;10(1):434-443
pubmed: 35492538
Sci Rep. 2019 Jun 25;9(1):9229
pubmed: 31239450
Integr Biol (Camb). 2011 Jun;3(6):653-62
pubmed: 21526262
J Biotechnol. 2006 Jan 2;121(1):86-101
pubmed: 16144726
Biomater Sci. 2019 Jul 23;7(8):3471-3479
pubmed: 31270512
J Am Chem Soc. 2006 Sep 20;128(37):12205-10
pubmed: 16967971
J Mater Chem B. 2017 Oct 28;5(40):8105-8114
pubmed: 32264649
Angew Chem Int Ed Engl. 2007;46(47):8970-4
pubmed: 17847154
Sci Adv. 2015 Oct 23;1(9):e1500758
pubmed: 26601312
Front Bioeng Biotechnol. 2021 Apr 12;9:637048
pubmed: 33912545
Tissue Eng Part A. 2020 Jan;26(1-2):93-101
pubmed: 31347470
Nature. 2006 Jul 27;442(7101):368-73
pubmed: 16871203
Biomater Sci. 2019 Feb 26;7(3):889-900
pubmed: 30608077
Acta Biomater. 2017 Nov;63:110-122
pubmed: 28919507
Regen Ther. 2016 Mar 01;3:24-31
pubmed: 31245469
Adv Mater. 2021 Dec;33(52):e2105361
pubmed: 34617338
ACS Biomater Sci Eng. 2016 Oct 10;2(10):1787-1795
pubmed: 33440476
Cancer Res. 1977 Oct;37(10):3639-43
pubmed: 908012
Adv Healthc Mater. 2019 Aug;8(15):e1900200
pubmed: 31190369
Adv Mater. 2012 Aug 16;24(31):4271-7
pubmed: 22740066
Stem Cells. 2017 Jan;35(1):51-60
pubmed: 27641724
Biomaterials. 2018 Oct;181:126-139
pubmed: 30081303
Trends Biotechnol. 2015 Nov;33(11):667-678
pubmed: 26603572
Biomicrofluidics. 2013 Aug 29;7(4):44130
pubmed: 24404063
Nat Biotechnol. 2017 Jul;35(7):640-646
pubmed: 28553940
ACS Appl Mater Interfaces. 2019 Feb 20;11(7):6867-6880
pubmed: 30676736
J Biomed Mater Res B Appl Biomater. 2017 Nov;105(8):2455-2464
pubmed: 27652573
Tissue Eng Part A. 2014 Apr;20(7-8):1134-44
pubmed: 24147855
Front Bioeng Biotechnol. 2019 Dec 20;7:420
pubmed: 31921823
Meat Sci. 2012 Nov;92(3):297-301
pubmed: 22543115
NPJ Sci Food. 2021 Mar 2;5(1):6
pubmed: 33654079
Kidney Blood Press Res. 2017;42(2):220-231
pubmed: 28478441
Front Chem. 2019 Jun 18;7:441
pubmed: 31275925
Adv Funct Mater. 2014 Jul 9;24(26):4060-4067
pubmed: 25411576
Sci Rep. 2015 Jun 11;5:11304
pubmed: 26065378
Biophys J. 2009 Jul 22;97(2):401-14
pubmed: 19619455
Nat Mater. 2013 Jun;12(6):584-90
pubmed: 23542870
Tissue Eng Part C Methods. 2015 Aug;21(8):852-61
pubmed: 25654582
Lab Chip. 2016 Dec 20;17(1):110-119
pubmed: 27869911
Regen Ther. 2017 Sep 11;7:52-60
pubmed: 30271852
Cell Death Dis. 2018 Aug 30;9(9):892
pubmed: 30166524
Pharmacol Ther. 2012 Apr;134(1):82-106
pubmed: 22252140
Anal Chem. 1998 Jun 1;70(11):2280-7
pubmed: 21644640
Phys Rev Lett. 2007 Aug 31;99(9):094502
pubmed: 17931011
Sci Rep. 2018 Jan 10;8(1):253
pubmed: 29321576
Adv Mater. 2015 Oct 7;27(37):5512-7
pubmed: 26283579
Lab Chip. 2020 Feb 7;20(3):490-495
pubmed: 31841123
Acta Biomater. 2014 Aug;10(8):3398-408
pubmed: 24681372
Tissue Eng Part A. 2018 Dec;24(23-24):1808-1817
pubmed: 29916333
Nat Commun. 2021 Feb 2;12(1):753
pubmed: 33531489
ACS Appl Mater Interfaces. 2015 Jun 17;7(23):12635-8
pubmed: 26040198
Transplantation. 2004 Feb 15;77(3):379-85
pubmed: 14966411
Curr Biol. 2017 Sep 11;27(17):R850-R852
pubmed: 28898649
Nano Lett. 2020 Apr 8;20(4):2257-2263
pubmed: 31751141
ACS Macro Lett. 2020 Sep 15;9(9):1369-1375
pubmed: 35638624
Lab Chip. 2012 Feb 7;12(3):422-33
pubmed: 22011791
Biomicrofluidics. 2018 Jan 10;12(1):014106
pubmed: 29375727
Lab Chip. 2008 Feb;8(2):287-93
pubmed: 18231668
Nat Commun. 2018 Mar 26;9(1):1222
pubmed: 29581433
Free Radic Biol Med. 2017 Dec;113:311-322
pubmed: 29032224
ACS Appl Mater Interfaces. 2019 Jul 3;11(26):22950-22961
pubmed: 31252493
Front Nutr. 2020 Feb 20;7:10
pubmed: 32154261
Mater Sci Eng R Rep. 2017 Sep;119:1-35
pubmed: 29200661