Reference Genome Assembly of the Big Berry Manzanita (Arctostaphylos glauca).
CCGP
California Conservation Genomics Project
California Floristic Province
chaparral
conservation
endemic
Journal
The Journal of heredity
ISSN: 1465-7333
Titre abrégé: J Hered
Pays: United States
ID NLM: 0375373
Informations de publication
Date de publication:
16 05 2022
16 05 2022
Historique:
received:
01
09
2021
accepted:
18
11
2021
entrez:
16
5
2022
pubmed:
17
5
2022
medline:
20
5
2022
Statut:
ppublish
Résumé
Arctostaphylos (Ericaceae) species, commonly known as manzanitas, are an invaluable fire-adapted chaparral clade in the California Floristic Province (CFP), a world biodiversity hotspot on the west coast of North America. This diverse woody genus includes many rare and/or endangered taxa, and the genus plays essential ecological roles in native ecosystems. Despite their importance in conservation management, and the many ecological and evolutionary studies that have focused on manzanitas, virtually no research has been conducted on the genomics of any manzanita species. Here, we report the first genome assembly of a manzanita species, the widespread Arctostaphylos glauca. Consistent with the genomics strategy of the California Conservation Genomics project, we used Pacific Biosciences HiFi long reads and Hi-C chromatin-proximity sequencing technology to produce a de novo assembled genome. The assembly comprises a total of 271 scaffolds spanning 547Mb, close to the genome size estimated by flow cytometry. This assembly, with a scaffold N50 of 31Mb and BUSCO complete score of 98.2%, will be used as a reference genome for understanding the genetic diversity and the basis of adaptations of both common and rare and endangered manzanita species.
Identifiants
pubmed: 35575079
pii: 6586230
doi: 10.1093/jhered/esab071
pmc: PMC9113465
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
188-196Subventions
Organisme : University of California, Riverside
Informations de copyright
© The American Genetic Association. 2021.
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