Genotyping of African Swine Fever Virus.
Gel electrophoresis
Genotyping
Polymerase chain reaction
Sequencing
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
16
5
2022
pubmed:
17
5
2022
medline:
20
5
2022
Statut:
ppublish
Résumé
Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral diseases of livestock. Real-time PCR (qPCR) is known as the gold standard diagnostic method for most diseases and is also used for the detection of African swine fever virus (ASFV) DNA in clinical specimens. To determine the ASFV genotype or identify additional genome markers, endpoint PCR is usually performed on ASFV-positive specimens, followed by Sanger sequencing and data analysis. Here, we describe the ASFV genotyping method used at the OIE Reference Laboratory for ASF at the Pirbright Institute, United Kingdom.
Identifiants
pubmed: 35575890
doi: 10.1007/978-1-0716-2333-6_8
doi:
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
119-132Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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