African Swine Fever Virus Plaque Assay and Disinfectant Testing.
African swine fever virus
Disinfectant
Plaque assay
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
16
5
2022
pubmed:
17
5
2022
medline:
20
5
2022
Statut:
ppublish
Résumé
Quantifying the titer of African swine fever virus is critical for disease control, viral infection studies, and disinfectant efficacy tests. Techniques such as real-time PCR and virus isolation provide an understanding as to whether viral genome is present or gives a qualitative assessment of live viral presence in a sample respectively, but neither provide a quantitative measurement of live virus. Here we describe a plaque assay for the titration of a Vero-adapted African swine fever virus strain (BA71V) and describe how to apply this method to determine disinfectant efficacy.
Identifiants
pubmed: 35575896
doi: 10.1007/978-1-0716-2333-6_14
doi:
Substances chimiques
Disinfectants
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
187-194Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Food and Agriculture Organisation of the United Nations (2021) ASF situation in Asia & Pacific Update. Retrieved from http://www.fao.org/ag/againfo/programmes/en/empres/ASF/Situation_update.html
Baer A, Kehn-Hall K (2014) Viral concentration determination through plaque assays: using traditional and novel overlay systems. J Vis Exp (93):e52065. Published 2014 Nov 4. https://doi.org/10.3791/52065
Enjuanes L, Carrascosa AL, Moreno MA et al (1976) Titration of African swine fever (ASF) virus. J Gen Virol 32(3):471–477. https://doi.org/10.1099/0022-1317-32-3-471
doi: 10.1099/0022-1317-32-3-471
pubmed: 823294
European Food Safety Authority (EFSA), Álvarez J, Bicout D, Boklund A et al (2019) Research gap analysis on African swine fever. EFSA J 17(8):e05811. https://doi.org/10.2903/j.efsa.2019.5811
doi: 10.2903/j.efsa.2019.5811