Metaeffector interactions modulate the type III effector-triggered immunity load of Pseudomonas syringae.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
07
12
2021
accepted:
21
04
2022
revised:
26
05
2022
pubmed:
17
5
2022
medline:
31
5
2022
entrez:
16
5
2022
Statut:
epublish
Résumé
The bacterial plant pathogen Pseudomonas syringae requires type III secreted effectors (T3SEs) for pathogenesis. However, a major facet of plant immunity entails the recognition of a subset of P. syringae's T3SEs by intracellular host receptors in a process called Effector-Triggered Immunity (ETI). Prior work has shown that ETI-eliciting T3SEs are pervasive in the P. syringae species complex raising the question of how P. syringae mitigates its ETI load to become a successful pathogen. While pathogens can evade ETI by T3SE mutation, recombination, or loss, there is increasing evidence that effector-effector (a.k.a., metaeffector) interactions can suppress ETI. To study the ETI-suppression potential of P. syringae T3SE repertoires, we compared the ETI-elicitation profiles of two genetically divergent strains: P. syringae pv. tomato DC3000 (PtoDC3000) and P. syringae pv. maculicola ES4326 (PmaES4326), which are both virulent on Arabidopsis thaliana but harbour largely distinct effector repertoires. Of the 529 T3SE alleles screened on A. thaliana Col-0 from the P. syringae T3SE compendium (PsyTEC), 69 alleles from 21 T3SE families elicited ETI in at least one of the two strain backgrounds, while 50 elicited ETI in both backgrounds, resulting in 19 differential ETI responses including two novel ETI-eliciting families: AvrPto1 and HopT1. Although most of these differences were quantitative, three ETI responses were completely absent in one of the pathogenic backgrounds. We performed ETI suppression screens to test if metaeffector interactions contributed to these ETI differences, and found that HopQ1a suppressed AvrPto1m-mediated ETI, while HopG1c and HopF1g suppressed HopT1b-mediated ETI. Overall, these results show that P. syringae strains leverage metaeffector interactions and ETI suppression to overcome the ETI load associated with their native T3SE repertoires.
Identifiants
pubmed: 35576228
doi: 10.1371/journal.ppat.1010541
pii: PPATHOGENS-D-21-02468
pmc: PMC9135338
doi:
Substances chimiques
Arabidopsis Proteins
0
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010541Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12638-43
pubmed: 10535975
PLoS Genet. 2010 Apr 01;6(4):e1000894
pubmed: 20368970
Mol Plant Microbe Interact. 2011 May;24(5):585-93
pubmed: 21198360
Annu Rev Phytopathol. 2013;51:85-104
pubmed: 23663005
Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2349-54
pubmed: 20133879
Plant Cell. 2010 Jan;22(1):260-72
pubmed: 20061552
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Plant Physiol. 2013 Apr;161(4):2062-74
pubmed: 23417089
Plant J. 2009 Nov;60(3):539-50
pubmed: 19624472
Mol Plant Pathol. 2018 Jul;19(7):1779-1794
pubmed: 29277959
Plant Cell. 1993 Aug;5(8):865-75
pubmed: 8400869
Mol Plant Microbe Interact. 2005 Apr;18(4):275-82
pubmed: 15828679
Cell. 2003 Feb 7;112(3):379-89
pubmed: 12581527
Nature. 2016 Nov 23;539(7630):524-529
pubmed: 27882964
EMBO J. 2000 Jul 3;19(13):3204-14
pubmed: 10880434
Plant Cell. 1996 Feb;8(2):251-257
pubmed: 12239384
Proc Natl Acad Sci U S A. 2005 May 3;102(18):6496-501
pubmed: 15845764
Mol Plant Microbe Interact. 2016 Dec;29(12):919-924
pubmed: 27996374
Mol Plant Pathol. 2017 Jan;18(1):152-168
pubmed: 27798954
Front Plant Sci. 2017 Dec 20;8:2157
pubmed: 29326748
Front Plant Sci. 2020 Aug 21;11:1290
pubmed: 32983191
Front Plant Sci. 2019 Apr 05;10:418
pubmed: 31024592
Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2975-80
pubmed: 21282655
Plant J. 2004 Feb;37(4):554-65
pubmed: 14756767
Curr Opin Plant Biol. 2021 Aug;62:102011
pubmed: 33677388
Plant J. 2018 Feb;93(4):651-663
pubmed: 29160935
Front Plant Sci. 2018 May 23;9:688
pubmed: 29875790
Plant J. 2014 Jan;77(2):235-45
pubmed: 24237140
Science. 2020 Feb 14;367(6479):763-768
pubmed: 32054757
Plant Cell. 2010 Jun;22(6):2033-44
pubmed: 20571112
Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10875-80
pubmed: 10485919
Nat Plants. 2017 Mar 13;3:17027
pubmed: 28288096
Cell. 2002 Mar 22;108(6):743-54
pubmed: 11955429
Nat Rev Genet. 2022 Feb 8;:
pubmed: 35136196
Gene. 1994 Oct 11;148(1):81-6
pubmed: 7926843
PLoS Pathog. 2021 Nov 1;17(11):e1010017
pubmed: 34724007
Mol Plant Microbe Interact. 2015 Jun;28(6):727-35
pubmed: 25625821
Hum Hered. 2010;70(2):132-40
pubmed: 20606458
Methods Mol Biol. 2015;1201:65-90
pubmed: 25388108
Mol Plant Pathol. 2020 Nov;21(11):1467-1480
pubmed: 32969167
Cell. 2003 Feb 7;112(3):369-77
pubmed: 12581526
Front Plant Sci. 2018 Jul 10;9:978
pubmed: 30042777
Genome Biol. 2019 Jan 3;20(1):3
pubmed: 30606234
Plant J. 2007 Jul;51(1):32-46
pubmed: 17559511
New Phytol. 2010 Sep;187(4):1018-1033
pubmed: 20636323
Int J Syst Evol Microbiol. 2007 Jan;57(Pt 1):81-91
pubmed: 17220447
Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2549-54
pubmed: 15701698
Microorganisms. 2021 May 11;9(5):
pubmed: 34064647
Cell Host Microbe. 2015 Jun 10;17(6):752-62
pubmed: 26067603
Science. 2002 Mar 1;295(5560):1722-6
pubmed: 11872842
Gene. 1977;2(2):95-113
pubmed: 344137
New Phytol. 2017 Jun;214(4):1673-1687
pubmed: 28295393
Gene. 1995 Dec 1;166(1):175-6
pubmed: 8529885
Cell. 2019 Sep 19;179(1):205-218.e21
pubmed: 31522888
Nature. 2006 Nov 16;444(7117):323-9
pubmed: 17108957
PLoS Pathog. 2010 Dec 02;6(12):e1001216
pubmed: 21151961
PLoS Pathog. 2008 Apr 04;4(4):e1000037
pubmed: 18389058
Mol Plant Microbe Interact. 2001 Feb;14(2):145-55
pubmed: 11204777
Plant Physiol. 2016 Jul;171(3):2239-55
pubmed: 27217495
PLoS Pathog. 2011 Mar;7(3):e1002010
pubmed: 21483484
Mol Plant Microbe Interact. 2009 Sep;22(9):1069-80
pubmed: 19656042
PLoS One. 2014 Dec 11;9(12):e114921
pubmed: 25503437
PLoS Pathog. 2013 Oct;9(10):e1003715
pubmed: 24204266
Cell Rep. 2015 Nov 24;13(8):1670-82
pubmed: 26586425
Pathogens. 2021 Jan 22;10(2):
pubmed: 33499048
Front Plant Sci. 2018 Aug 14;9:977
pubmed: 30154802
Nat Rev Microbiol. 2018 May;16(5):316-328
pubmed: 29479077
Cell Rep. 2018 May 8;23(6):1630-1638
pubmed: 29742421
J Biol Chem. 2011 Apr 22;286(16):14129-36
pubmed: 21378171