Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
16 05 2022
16 05 2022
Historique:
received:
08
02
2022
accepted:
26
04
2022
entrez:
16
5
2022
pubmed:
17
5
2022
medline:
20
5
2022
Statut:
epublish
Résumé
In this work we present the potential of Natural Deep Eutectic Systems (NADES) as new vitrification media for the cryopreservation of mammalian cells. Several NADES composed of natural metabolites were prepared and tested as CPAs in two cell lines, L929 and HacaT cells. After the harvesting, cells were mixed with the eutectic systems, and frozen directly into liquid nitrogen to achieve a vitreous state. Then, the cells were thawed and it was observed that NADES were able to exert a significant cryoprotective effect in L929 cells, when compared with DMSO or in the absence of a CPA. For HacaT cells, only a eutectic system showed a slightly improvement in cell survival, while DMSO caused complete cell death. Moreover, the thermal behaviour of the best systems was studied for further understanding the protective properties of NADES as CPAs, and have shown a significant difference in terms of T
Identifiants
pubmed: 35577888
doi: 10.1038/s41598-022-12365-4
pii: 10.1038/s41598-022-12365-4
pmc: PMC9110728
doi:
Substances chimiques
Cryoprotective Agents
0
Dimethyl Sulfoxide
YOW8V9698H
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8095Subventions
Organisme : European Research Council
ID : ERC-2016-CoG 725034
Pays : International
Informations de copyright
© 2022. The Author(s).
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