The effect of BMP4, FGF8 and WNT3a on mouse iPS cells differentiating to odontoblast-like cells.
BMP4
Differentiation
FGF8
Mouse iPS cells
Odontoblasts
WNT3a
Journal
Medical molecular morphology
ISSN: 1860-1499
Titre abrégé: Med Mol Morphol
Pays: Japan
ID NLM: 101239023
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
24
01
2022
accepted:
23
03
2022
pubmed:
17
5
2022
medline:
17
8
2022
entrez:
16
5
2022
Statut:
ppublish
Résumé
We investigated whether BMP4, FGF8, and/or WNT3a on neural crest-like cells (NCLC) derived from mouse induced pluripotent stem (miPS) cells will promote differentiation of odontoblasts-like cells. After the miPS cells matured into embryonic body (EB) cells, they were cultured in a neural induction medium to produce NCLC. As the differentiation of NCLC were confirmed by RT-qPCR, they were then disassociated and cultured with a medium containing, BMP4, FGF8, and/or WNT3a for 7 and 14 days. The effect of these stimuli on NCLC were assessed by RT-qPCR, ALP staining, and immunocytochemistry. The cultured EB cells presented a significant increase of Snai1, Slug, and Sox 10 substantiating the differentiation of NCLC. NCLC stimulated with more than two stimuli significantly increased the odontoblast markers Dmp-1, Dspp, Nestin, Alp, and Runx2 expression compared to control with no stimulus. The expression of Dmp-1 and Dspp upregulated more when FGF8 was combined with WNT3a. ALP staining was positive in groups containing BMP4 and fluorescence was observed in immunocytochemistry of the common significant groups between Dmp-1 and Dspp. After stimulation, the cell morphology demonstrated a spindle-shaped cells with long projections resembling odontoblasts. Simultaneous BMP4, FGF8, and WNT3a stimuli significantly differentiated NCLC into odontoblast-like cells.
Identifiants
pubmed: 35578118
doi: 10.1007/s00795-022-00318-9
pii: 10.1007/s00795-022-00318-9
doi:
Substances chimiques
Bmp4 protein, mouse
0
Bone Morphogenetic Protein 4
0
Fgf8 protein, mouse
0
Wnt3A Protein
0
Wnt3a protein, mouse
0
Fibroblast Growth Factor 8
148997-75-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
199-209Subventions
Organisme : Ministry of Science and Technology
ID : KIBANKENNKYU B 18H03007
Organisme : Ministry of Science and Technology
ID : KIBANKENNKYU C 18K09753
Organisme : Ministry of Science and Technology
ID : KIBANKENNKYU C 19K10063
Organisme : Ministry of Science and Technology
ID : 19K19247
Informations de copyright
© 2022. The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology.
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