Graphite nanocrystals coated paper-based electrode for detection of SARS-Cov-2 gene using DNA-functionalized Au@carbon dot core-shell nanoparticles.
Au@CD core–shell NPs
Electrochemical genosensor
Graphite nanocrystals
SARS-CoV-2 RdRP
Journal
Microchemical journal : devoted to the application of microtechniques in all branches of science
ISSN: 0026-265X
Titre abrégé: Microchem J
Pays: United States
ID NLM: 0376647
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
received:
23
03
2022
revised:
09
05
2022
accepted:
10
05
2022
entrez:
17
5
2022
pubmed:
18
5
2022
medline:
18
5
2022
Statut:
ppublish
Résumé
Currently, the development of biosensors is an urgent need due to the rapid spread of SARS-CoV-2 and the limitations of current standard methods for the diagnosis of COVID-19. Hence, many researchers have focused on the design of high-performance biosensors for measuring coronavirus genes. In this study, a voltammetric genosensor was developed for the determination of SARS-CoV-2 RdRP gene based on the format of cDNA probe/Au@CD core-shell NPs/graphite nanocrystals (GNCs)/paper electrode. For the first time, graphite nanocrystals were used in the electrochemical biosensor design. This genosensor was exposed to different concentrations of virus gene and then the hybridization between cDNA probe and RdRP gene was monitored by redox-active toluidine blue (TB). With increasing the RdRP concentration, the reduction peak current of TB enhanced in a linear range of 0.50 pM-12.00 nM according to the regression equation of I (μA) = 7.60 log C
Identifiants
pubmed: 35578710
doi: 10.1016/j.microc.2022.107585
pii: S0026-265X(22)00413-1
pmc: PMC9093088
doi:
Types de publication
Journal Article
Langues
eng
Pagination
107585Informations de copyright
© 2022 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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