Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria.
FtsZ assembly
bacterial cell division
bottom-up synthetic biology
dendrimersomes
dynamic Min patterns
synthetic cells
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
revised:
11
04
2022
received:
14
03
2022
pubmed:
18
5
2022
medline:
16
7
2022
entrez:
17
5
2022
Statut:
ppublish
Résumé
The integration of active cell machinery with synthetic building blocks is the bridge toward developing synthetic cells with biological functions and beyond. Self-replication is one of the most important tasks of living systems, and various complex machineries exist to execute it. In Escherichia coli, a contractile division ring is positioned to mid-cell by concentration oscillations of self-organizing proteins (MinCDE), where it severs membrane and cell wall. So far, the reconstitution of any cell division machinery has exclusively been tied to liposomes. Here, the reconstitution of a rudimentary bacterial divisome in fully synthetic bicomponent dendrimersomes is shown. By tuning the membrane composition, the interaction of biological machinery with synthetic membranes can be tailored to reproduce its dynamic behavior. This constitutes an important breakthrough in the assembly of synthetic cells with biological elements, as tuning of membrane-divisome interactions is the key to engineering emergent biological behavior from the bottom-up.
Identifiants
pubmed: 35579491
doi: 10.1002/adma.202202364
doi:
Substances chimiques
Bacterial Proteins
0
Escherichia coli Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2202364Subventions
Organisme : H2020-NMBP-TR-IND-2018, EVPRO (Development of Extracellular Vesicles loaded hydrogel coatings with immunomodulatory activity for Promoted Regenerative Osseointegration of revision endoprosthesis)
ID : 814495-2
Organisme : Max Planck Bristol Centre for Minimal Biology, the Max Planck Society
Organisme : ERS Seed Fund from RWTH Aachen University
ID : SFSynt003
Organisme : ERS Seed Fund from RWTH Aachen University
ID : SFSynt005
Organisme : CERCA programme
Organisme : Generalitat de Catalunya
ID : 2017-SGR-1442
Organisme : Biomedical Research Networking Center (CIBER), Spain
Organisme : National Agency for Research and Development (ANID) / Scholarship Program Postdoctorado becas
ID : Chile/2018 - 74190117
Informations de copyright
© 2022 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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