Origins of glycan selectivity in streptococcal Siglec-like adhesins suggest mechanisms of receptor adaptation.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 05 2022
18 05 2022
Historique:
received:
10
04
2021
accepted:
26
04
2022
entrez:
18
5
2022
pubmed:
19
5
2022
medline:
21
5
2022
Statut:
epublish
Résumé
Bacterial binding to host receptors underlies both commensalism and pathogenesis. Many streptococci adhere to protein-attached carbohydrates expressed on cell surfaces using Siglec-like binding regions (SLBRs). The precise glycan repertoire recognized may dictate whether the organism is a strict commensal versus a pathogen. However, it is currently not clear what drives receptor selectivity. Here, we use five representative SLBRs and identify regions of the receptor binding site that are hypervariable in sequence and structure. We show that these regions control the identity of the preferred carbohydrate ligand using chimeragenesis and single amino acid substitutions. We further evaluate how the identity of the preferred ligand affects the interaction with glycoprotein receptors in human saliva and plasma samples. As point mutations can change the preferred human receptor, these studies suggest how streptococci may adapt to changes in the environmental glycan repertoire.
Identifiants
pubmed: 35585145
doi: 10.1038/s41467-022-30509-y
pii: 10.1038/s41467-022-30509-y
pmc: PMC9117288
doi:
Substances chimiques
Adhesins, Bacterial
0
Ligands
0
Polysaccharides
0
Sialic Acid Binding Immunoglobulin-like Lectins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2753Subventions
Organisme : NIAID NIH HHS
ID : R01 AI106987
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103393
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI130684
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103694
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM137458
Pays : United States
Organisme : NIGMS NIH HHS
ID : R24 GM098791
Pays : United States
Organisme : NIDCR NIH HHS
ID : R03 DE029516
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA221244
Pays : United States
Informations de copyright
© 2022. The Author(s).
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