Fluorescence Studies: A9 Peptide, Functionalized with a Fluorogenic Probe, Interacts with Its Receptor Model HER2-DIVMP.
Journal
ACS medicinal chemistry letters
ISSN: 1948-5875
Titre abrégé: ACS Med Chem Lett
Pays: United States
ID NLM: 101521073
Informations de publication
Date de publication:
12 May 2022
12 May 2022
Historique:
received:
17
01
2022
accepted:
04
04
2022
entrez:
19
5
2022
pubmed:
20
5
2022
medline:
20
5
2022
Statut:
epublish
Résumé
A recently developed synthetic protocol allowed for the functionalization of the active peptide A9 with a fluorogenic probe, which is useful for studying biomolecular interactions. Essentially, a nucleophilic attack on a halo-substituted benzofurazan is selectively performed by a cysteine sulfhydryl group. The process is assisted by the basic catalysis of activated zeolites (4 Å molecular sieves) and promoted by microwave irradiation. Fluorescence studies revealed that a donor-acceptor pair within the peptide sequence was introduced, thus allowing a deeper investigation on the interaction process between the peptide ligand and its receptor fragment. The obtained results allowed us to come full circle for all the currently understood structural determinants that were found to be involved in the binding process.
Identifiants
pubmed: 35586438
doi: 10.1021/acsmedchemlett.2c00026
pmc: PMC9109265
doi:
Types de publication
Journal Article
Langues
eng
Pagination
807-811Informations de copyright
© 2022 American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
Références
Nat Methods. 2019 Sep;16(9):815-829
pubmed: 31471616
Eur J Med Chem. 2013 Mar;61:116-21
pubmed: 23107515
Eur J Med Chem. 2015 Jan 7;89:156-71
pubmed: 25462236
Biopolymers. 2000-2001;56(1):47-53
pubmed: 11582577
Bioorg Med Chem. 2009 Oct 1;17(19):7015-20
pubmed: 19733086
Mol Biosyst. 2016 Jun 21;12(7):2159-67
pubmed: 27113673
Amino Acids. 2014 May;46(5):1197-206
pubmed: 24493095
Sci Rep. 2018 Feb 14;8(1):2998
pubmed: 29445216
Amino Acids. 2015 Aug;47(8):1507-15
pubmed: 25900810
Amino Acids. 2016 Sep;48(9):2267-71
pubmed: 27351201
Org Lett. 2015 Nov 20;17(22):5646-9
pubmed: 26523342
J Pept Sci. 2020 Feb;26(2):e3231
pubmed: 31749266
Chem Pharm Bull (Tokyo). 2020;68(10):954-961
pubmed: 32999147
J Med Chem. 2020 Dec 24;63(24):15333-15343
pubmed: 33226807