Fast raster-scan optoacoustic mesoscopy enables assessment of human melanoma microvasculature in vivo.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
19 05 2022
Historique:
received: 22 06 2021
accepted: 03 05 2022
entrez: 19 5 2022
pubmed: 20 5 2022
medline: 24 5 2022
Statut: epublish

Résumé

Melanoma is associated with angiogenesis and vascular changes that may extend through the entire skin depth. Three-dimensional imaging of vascular characteristics in skin lesions could therefore allow diagnostic insights not available by conventional visual inspection. Raster-scan optoacoustic mesoscopy (RSOM) images microvasculature through the entire skin depth with resolutions of tens of micrometers; however, current RSOM implementations are too slow to overcome the strong breathing motions on the upper torso where melanoma lesions commonly occur. To enable high-resolution imaging of melanoma vasculature in humans, we accelerate RSOM scanning using an illumination scheme that is coaxial with a high-sensitivity ultrasound detector path, yielding 15 s single-breath-hold scans that minimize motion artifacts. We apply this Fast RSOM to image 10 melanomas and 10 benign nevi in vivo, showing marked differences between malignant and benign lesions, supporting the possibility to use biomarkers extracted from RSOM imaging of vasculature for lesion characterization to improve diagnostics.

Identifiants

pubmed: 35589757
doi: 10.1038/s41467-022-30471-9
pii: 10.1038/s41467-022-30471-9
pmc: PMC9120110
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2803

Informations de copyright

© 2022. The Author(s).

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Auteurs

Hailong He (H)

Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany.

Christine Schönmann (C)

Department of Dermatology and Allergy, Technical University of Munich, Munich, Germany.

Mathias Schwarz (M)

iThera Medical GmbH, Munich, Germany.

Benedikt Hindelang (B)

Department of Dermatology and Allergy, Technical University of Munich, Munich, Germany.

Andrei Berezhnoi (A)

Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany.

Susanne Annette Steimle-Grauer (SA)

Department of Dermatology and Allergy, Technical University of Munich, Munich, Germany.

Ulf Darsow (U)

Department of Dermatology and Allergy, Technical University of Munich, Munich, Germany.

Juan Aguirre (J)

Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany.

Vasilis Ntziachristos (V)

Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany. bioimaging.translatum@tum.de.
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany. bioimaging.translatum@tum.de.

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