Laser coagulation and hemostasis of large diameter blood vessels: effect of shear stress and flow velocity.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
19 05 2022
Historique:
received: 24 10 2021
accepted: 29 04 2022
entrez: 19 5 2022
pubmed: 20 5 2022
medline: 24 5 2022
Statut: epublish

Résumé

Photocoagulation of blood vessels offers unambiguous advantages to current radiofrequency approaches considering the high specificity of blood absorption at available laser wavelengths (e.g., 532 nm and 1.064 µm). Successful treatment of pediatric vascular lesions, such as port-wine stains requiring microvascular hemostasis, has been documented. Although laser treatments have been successful in smaller diameter blood vessels, photocoagulation of larger sized vessels is less effective. The hypothesis for this study is that a primary limitation in laser coagulation of large diameter blood vessels (500-1000 µm) originates from shear stress gradients associated with higher flow velocities along with temperature-dependent viscosity changes. Laser (1.07 µm) coagulation of blood vessels was tested in the chicken chorio-allantoic membrane (CAM). A finite element model is developed that includes hypothetical limitations in laser coagulation during irradiation. A protocol to specify laser dosimetry is derived from OCT imaging and angiography observations as well as finite element model results. Laser dosimetry is applied in the CAM model to test the experimental hypothesis that blood shear stress and flow velocity are important parameters for laser coagulation and hemostasis of large diameter blood vessels (500-1000 µm). Our experimental results suggest that shear stress and flow velocity are fundamental in the coagulation of large diameter blood vessels (500-1000 µm). Laser dosimetry is proposed and demonstrated for successful coagulation and hemostasis of large diameter CAM blood vessels.

Identifiants

pubmed: 35589781
doi: 10.1038/s41598-022-12128-1
pii: 10.1038/s41598-022-12128-1
pmc: PMC9120470
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

8375

Informations de copyright

© 2022. The Author(s).

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Auteurs

Nitesh Katta (N)

University of California at Irvine, Irvine, CA, USA. nkatta@uci.edu.

Daniel Santos (D)

The University of Texas at Austin, Austin, TX, USA.

Austin B McElroy (AB)

The University of Texas at Austin, Austin, TX, USA.

Arnold D Estrada (AD)

The University of Texas at Austin, Austin, TX, USA.

Glori Das (G)

The University of Texas at Austin, Austin, TX, USA.

Mohammad Mohsin (M)

University of Connecticut, Storrs, CT, USA.

Moses Donovan (M)

Pennsylvania State University, State College, PA, USA.

Thomas E Milner (TE)

University of California at Irvine, Irvine, CA, USA.

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