A Novel PCR-Based Methodology for Viral Detection Utilizing Mechanical Homogenization.

The impact of viral diseases on human health is becoming increasingly prevalent globally with the burden of disease being shared between resource-rich and poor areas. As seen in the global pandemic caused by SARS-CoV-2, there is a need to establish viral detection techniques applicable to resource-limited areas that provide sensitive and specific testing with a logistically conscious mindset. Herein, we describe a direct-to-PCR technology utilizing mechanical homogenization prior to viral PCR detection, which allows the user to bypass traditional RNA extraction techniques for accurate detection of human coronavirus. This methodology was validated

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Déclaration de conflit d'intérêts

Competing interestsZPM, CMP, and RJN are named inventors on the patent describing this method held by Omni International, A PerkinElmer Company, but have no personal financial interests to disclose in the direct commercialization of this technology. CMP, GLR, and RJN are all full-time employees of Omni International Inc, A PerkinElmer Company, and GLR and RJN have no personal financial interests in the performance of the company. CMP is a shareholder of PerkinElmer, holding a personal financial interest in the performance of the company. ZPM is in a consulting relationship with Omni International, A PerkinElmer Company and holds no personal financial interests in the performance of the company. ZPM and RJN are also associated with Jeevan Biosciences LLC, a company which is not involved in the work described within this manuscript.