HIV-associated vaginal microbiome and inflammation predict spontaneous preterm birth in Zambia.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
20 05 2022
20 05 2022
Historique:
received:
25
01
2022
accepted:
26
04
2022
entrez:
20
5
2022
pubmed:
21
5
2022
medline:
25
5
2022
Statut:
epublish
Résumé
A Lactobacillus-deficient, anaerobe-rich vaginal microbiome has been associated with local inflammation and spontaneous preterm birth (sPTB), but few studies have assessed this association in the setting of HIV. We performed metagenomic sequencing and inflammatory marker assays on vaginal swabs collected in pregnancy. We grouped samples into 7 metagenomic clusters (mgClust) using the non-redundant VIRGO catalogue, and derived inflammatory scores by factor analysis. Of 221 participants, median Shannon diversity index (SDI) was highest in HIV+ with detectable viral load (1.31, IQR: 0.85-1.66; p < 0.001) and HIV+ with undetectable virus (1.17, IQR: 0.51-1.66; p = 0.01) compared to HIV- (0.74, IQR: 0.35-1.26). Inflammatory scores positively correlated with SDI (+ 0.66, 95%CI 0.28, 1.03; p = 0.001), highest among anaerobe-rich mgClust2-mgClust6. HIV was associated with predominance of anaerobe-rich mgClust5 (17% vs. 6%; p = 0.02) and mgClust6 (27% vs. 11%; p = 0.002). Relative abundance of a novel Gardnerella metagenomic subspecies > 50% predicted sPTB (RR 2.6; 95%CI: 1.1, 6.4) and was higher in HIV+ (23% vs. 10%; p = 0.001). A novel Gardnerella metagenomic subspecies more abundant in women with HIV predicted sPTB. The risk of sPTB among women with HIV may be mediated by the vaginal microbiome and inflammation, suggesting potential targets for prevention.
Identifiants
pubmed: 35595739
doi: 10.1038/s41598-022-12424-w
pii: 10.1038/s41598-022-12424-w
pmc: PMC9123167
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8573Subventions
Organisme : FIC NIH HHS
ID : K01 TW010857
Pays : United States
Organisme : NIH HHS
ID : P30 AI50410; T32 HD075731; K01 TW010857; D43 TW009340; R21DK123674; R01HL148672
Pays : United States
Organisme : Bill & Melinda Gates Foundation
ID : OPP1033514
Pays : United States
Organisme : The Gerber Foundation
ID : 6361
Pays : United States
Organisme : Bill & Melinda Gates Foundation
ID : OPP1189217
Pays : United States
Informations de copyright
© 2022. The Author(s).
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