A road map for in vivo evolution experiments with blood-borne parasitic microbes.
Bartonella spp.
experimental evolution
host-parasite adaptation
in vivo experiments
microbial pathogens
population bottlenecks
sequential passages
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
revised:
14
03
2022
received:
07
11
2021
accepted:
13
05
2022
pubmed:
24
5
2022
medline:
5
10
2022
entrez:
23
5
2022
Statut:
ppublish
Résumé
Laboratory experiments in which blood-borne parasitic microbes evolve in their animal hosts offer an opportunity to study parasite evolution and adaptation in real time and under natural settings. The main challenge of these experiments is to establish a protocol that is both practical over multiple passages and accurately reflects natural transmission scenarios and mechanisms. We provide a guide to the steps that should be considered when designing such a protocol, and we demonstrate its use via a case study. We highlight the importance of choosing suitable ancestral genotypes, treatments, number of replicates per treatment, types of negative controls, dependent variables, covariates, and the timing of checkpoints for the experimental design. We also recommend specific preliminary experiments to determine effective methods for parasite quantification, transmission, and preservation. Although these methodological considerations are technical, they also often have conceptual implications. To this end, we encourage other researchers to design and conduct in vivo evolution experiments with blood-borne parasitic microbes, despite the challenges that the work entails.
Identifiants
pubmed: 35599628
doi: 10.1111/1755-0998.13649
pmc: PMC9796859
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
2843-2859Subventions
Organisme : Ecology of Infectious Diseases grant from the National Science foundation under the auspices of U.S.-Israel Binational Science Foundation (NSF-BSF)
ID : DEB-1813069
Organisme : The Israel Science Foundation (ISF)
ID : 1391/15
Informations de copyright
© 2022 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.
Références
AIMS Microbiol. 2018 Mar 1;4(1):140-164
pubmed: 31294207
J Infect Dis. 2006 Feb 1;193(3):380-6
pubmed: 16388485
BMC Biol. 2012 Dec 18;10:104
pubmed: 23249484
Microbiol Mol Biol Rev. 2018 Jul 25;82(3):
pubmed: 30045954
Mol Ecol Resour. 2022 Nov;22(8):2843-2859
pubmed: 35599628
Comp Immunol Microbiol Infect Dis. 2010 Mar;33(2):95-107
pubmed: 20097421
Microb Pathog. 2003 May;34(5):239-48
pubmed: 12732472
Microbiol Spectr. 2017 Sep;5(5):
pubmed: 28884670
J Wildl Dis. 1999 Apr;35(2):275-84
pubmed: 10231754
Mol Ecol. 2012 May;21(9):2058-77
pubmed: 22332770
Trends Ecol Evol. 2013 Jun;28(6):367-75
pubmed: 23523051
Infect Immun. 1998 Nov;66(11):5534-6
pubmed: 9784568
Front Microbiol. 2018 May 08;9:921
pubmed: 29867832
Virus Evol. 2019 Aug 02;5(2):vez024
pubmed: 31768264
BMC Infect Dis. 2012 Mar 28;12:71
pubmed: 22455545
Science. 2018 Nov 9;362(6415):
pubmed: 30409860
PLoS Pathog. 2011 Nov;7(11):e1002335
pubmed: 22102808
Exp Parasitol. 2020 Feb 21;211:107861
pubmed: 32112944
Antimicrob Agents Chemother. 2000 Aug;44(8):2118-25
pubmed: 10898684
Int J Parasitol. 2005 Feb;35(2):145-53
pubmed: 15710435
Pathogens. 2020 Mar 12;9(3):
pubmed: 32178297
PLoS Genet. 2018 Apr 27;14(4):e1007324
pubmed: 29702649
PLoS Pathog. 2021 Sep 9;17(9):e1009872
pubmed: 34499699
Am J Hyg. 1952 Jan;55(1):108-24
pubmed: 14885170
J Appl Microbiol. 2017 Aug;123(2):464-477
pubmed: 28600831
Microbes Infect. 2012 May;14(5):457-69
pubmed: 22230246
J Evol Biol. 2011 Jun;24(6):1307-16
pubmed: 21481055
Am Nat. 2017 Aug;190(S1):S57-S68
pubmed: 28731830
J Clin Microbiol. 1996 Jun;34(6):1429-32
pubmed: 8735093
Mol Biol Evol. 2012 Jan;29(1):391-400
pubmed: 21965342
Nat Rev Genet. 2013 Dec;14(12):827-39
pubmed: 24166031
Sci Rep. 2020 Oct 27;10(1):18404
pubmed: 33110109
J Evol Biol. 2018 Feb;31(2):314-322
pubmed: 29266576
Infect Immun. 2007 Jan;75(1):35-43
pubmed: 17060468
Trop Parasitol. 2014 Jul;4(2):80-9
pubmed: 25250227
Genetics. 2002 Oct;162(2):961-71
pubmed: 12399403
Nat Rev Microbiol. 2012 Mar 27;10(5):352-65
pubmed: 22450379
Virology. 2007 Apr 10;360(2):469-76
pubmed: 17134731
J Virol. 2021 Feb 3;:
pubmed: 33536175
Trends Ecol Evol. 2012 Oct;27(10):547-60
pubmed: 22819306
Mol Biol Evol. 2015 May;32(5):1132-47
pubmed: 25660377
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6808-14
pubmed: 8041701
Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):2112-2117
pubmed: 30659157
Proc Biol Sci. 2015 Jan 7;282(1798):20141568
pubmed: 25392466
Genome Biol Evol. 2018 Nov 1;10(11):3089-3103
pubmed: 30346520
Proc Natl Acad Sci U S A. 2008 May 13;105(19):6970-5
pubmed: 18458341
mBio. 2014 May 06;5(3):e00966-14
pubmed: 24803516
Mol Biol Evol. 2010 Apr;27(4):819-32
pubmed: 19955476
Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):7899-906
pubmed: 18524956
PLoS Pathog. 2021 Jun 24;17(6):e1009643
pubmed: 34166469
Annu Rev Virol. 2020 Sep 29;7(1):63-81
pubmed: 32511081
Front Microbiol. 2018 Jul 03;9:1279
pubmed: 30018594
Clin Vaccine Immunol. 2011 Nov;18(11):1962-8
pubmed: 21880854
Mol Ecol. 2018 Dec;27(23):4787-4807
pubmed: 30357977
Mol Ecol. 2011 Mar;20(5):972-80
pubmed: 20874764
Ticks Tick Borne Dis. 2021 Jul;12(4):101693
pubmed: 33690089
Virology. 2008 May 10;374(2):256-60
pubmed: 18395240
Microbiol Immunol. 2018 Jan;62(1):24-33
pubmed: 29171073
J Evol Biol. 2016 Dec;29(12):2545-2555
pubmed: 27575521
Proc Natl Acad Sci U S A. 2010 Jan 19;107(3):1154-9
pubmed: 20080608
Lab Anim. 2001 Jan;35(1):1-41
pubmed: 11201285
PLoS One. 2009 Nov 17;4(11):e7876
pubmed: 19924238
Proc Natl Acad Sci U S A. 2005 May 24;102(21):7624-8
pubmed: 15894623
Cell. 2020 Feb 6;180(3):454-470.e18
pubmed: 32004459
ISME J. 2017 Oct;11(10):2181-2194
pubmed: 28509909
PLoS Biol. 2007 Sep;5(9):e225
pubmed: 17713986
Mol Ecol. 2011 Jul;20(13):2864-70
pubmed: 21692752
Front Microbiol. 2020 Aug 11;11:1889
pubmed: 32849468
Ecol Lett. 2013 Apr;16(4):556-67
pubmed: 23347009
Evolution. 2019 Dec;73(12):2461-2475
pubmed: 31433508
Evolution. 1999 Jun;53(3):689-703
pubmed: 28565637
J Infect Dis. 2017 Jan 15;215(2):303-311
pubmed: 27803173
Vet Immunol Immunopathol. 1993 May;36(4):369-78
pubmed: 8333145
Exp Parasitol. 2008 Dec;120(4):372-80
pubmed: 18809402
Proc Natl Acad Sci U S A. 2005 Aug 23;102(34):12112-6
pubmed: 16099836
Front Microbiol. 2016 Sep 13;7:1444
pubmed: 27679620
PLoS Pathog. 2020 Jan 17;16(1):e1008191
pubmed: 31951644
Vet Microbiol. 2007 Jun 21;122(3-4):290-7
pubmed: 17321078
Appl Environ Microbiol. 2012 Aug;78(16):5646-52
pubmed: 22660719
PLoS Biol. 2018 Dec 10;16(12):e2006893
pubmed: 30532251
J Exp Biol. 2019 Jul 31;222(Pt 15):
pubmed: 31285244
Science. 1998 Nov 20;282(5393):1432-5
pubmed: 9822369
J Antibiot (Tokyo). 2018 Feb;71(2):279-286
pubmed: 28928474
Mol Ecol. 2017 Apr;26(7):1703-1710
pubmed: 28409900
Science. 2020 Oct 23;370(6515):476-478
pubmed: 33093112