Search for morphological indicators that predict implantation by principal component analysis using images of blastocyst.


Journal

PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425

Informations de publication

Date de publication:
2022
Historique:
received: 23 02 2022
accepted: 25 04 2022
entrez: 23 5 2022
pubmed: 24 5 2022
medline: 24 5 2022
Statut: epublish

Résumé

Although the current evaluation of human blastocysts is based on the Gardner criteria, there may be other notable parameters. The purpose of our study was to clarify whether the morphology of blastocysts has notable indicators other than the Gardner criteria. To find such indicators, we compared blastocysts that showed elevated human chorionic gonadotropin (hCG) levels after transplantation (hCG-positive group; The comparison revealed that the hCG-positive group had grainy morphology and the hCG-negative group had non-grainy morphology. Classification of the blastocysts by this indicator did not make a difference in Gardner score. Interestingly, all embryos with ≥20% fragmentation were non-grainy. The visual classification based on this analysis was significantly more accurate than the prediction of implantation using the Gardner score ≥3BB. As graininess can be used in combination with the Gardner score, this indicator will enhance current reproductive technologies.

Sections du résumé

Background
Although the current evaluation of human blastocysts is based on the Gardner criteria, there may be other notable parameters. The purpose of our study was to clarify whether the morphology of blastocysts has notable indicators other than the Gardner criteria.
Methods
To find such indicators, we compared blastocysts that showed elevated human chorionic gonadotropin (hCG) levels after transplantation (hCG-positive group;
Results
The comparison revealed that the hCG-positive group had grainy morphology and the hCG-negative group had non-grainy morphology. Classification of the blastocysts by this indicator did not make a difference in Gardner score. Interestingly, all embryos with ≥20% fragmentation were non-grainy. The visual classification based on this analysis was significantly more accurate than the prediction of implantation using the Gardner score ≥3BB. As graininess can be used in combination with the Gardner score, this indicator will enhance current reproductive technologies.

Identifiants

pubmed: 35602891
doi: 10.7717/peerj.13441
pii: 13441
pmc: PMC9119295
doi:

Substances chimiques

Chorionic Gonadotropin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

e13441

Informations de copyright

© 2022 Mashiko et al.

Déclaration de conflit d'intérêts

The authors declare that they have no competing interests.

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Auteurs

Daisuke Mashiko (D)

Graduate School of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.

Mikiko Tokoro (M)

Graduate School of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.
Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Nagoya, Aichi, Japan.

Masae Kojima (M)

Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Nagoya, Aichi, Japan.

Noritaka Fukunaga (N)

Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Nagoya, Aichi, Japan.

Yoshimasa Asada (Y)

Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Nagoya, Aichi, Japan.

Kazuo Yamagata (K)

Graduate School of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.

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