A Case Study to Dissect Immunity to SARS-CoV-2 in a Neonate Nonhuman Primate Model.
COVID-19
SARS-CoV-2
children
innate immunity
microbiota
neonate
nonhuman primate
pediatric
type-I IFN
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2022
2022
Historique:
received:
14
01
2022
accepted:
05
04
2022
entrez:
23
5
2022
pubmed:
24
5
2022
medline:
25
5
2022
Statut:
epublish
Résumé
Most children are less severely affected by coronavirus-induced disease 2019 (COVID-19) than adults, and thus more difficult to study progressively. Here, we provide a neonatal nonhuman primate (NHP) deep analysis of early immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in blood and mucosal tissues. In addition, we provide a comparison with SARS-CoV-2-infected adult NHP. Infection of the neonate resulted in a mild disease compared with adult NHPs that develop, in most cases, moderate lung lesions. In concomitance with the viral RNA load increase, we observed the development of an early innate response in the blood, as demonstrated by RNA sequencing, flow cytometry, and cytokine longitudinal data analyses. This response included the presence of an antiviral type-I IFN gene signature, a persistent and lasting NKT cell population, a balanced peripheral and mucosal IFN-γ/IL-10 cytokine response, and an increase in B cells that was accompanied with anti-SARS-CoV-2 antibody response. Viral kinetics and immune responses coincided with changes in the microbiota profile composition in the pharyngeal and rectal mucosae. In the mother, viral RNA loads were close to the quantification limit, despite the very close contact with SARS-CoV-2-exposed neonate. This pilot study demonstrates that neonatal NHPs are a relevant model for pediatric SARS-CoV-2 infection, permitting insights into the early steps of anti-SARS-CoV-2 immune responses in infants.
Identifiants
pubmed: 35603150
doi: 10.3389/fimmu.2022.855230
pmc: PMC9114777
doi:
Substances chimiques
Cytokines
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
855230Informations de copyright
Copyright © 2022 Fovet, Pimienta, Galhaut, Relouzat, Nunez, Cavarelli, Sconosciuti, Dhooge, Marzinotto, Lampasona, Tolazzi, Scarlatti, Ho Tsong Fang, Naninck, Dereuddre-Bosquet, Van Wassenhove, Gallouët, Maisonnasse, Le Grand, Menu and Seddiki.
Déclaration de conflit d'intérêts
Author Natalia Nunez was employed by Life and Soft, Fontenay-aux-Roses, France. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Front Immunol. 2021 Nov 10;12:750279
pubmed: 34858406
Science. 2020 Dec 11;370(6522):1339-1343
pubmed: 33159009
JAMA Pediatr. 2020 Sep 1;174(9):902-903
pubmed: 32745201
Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6771-6776
pubmed: 32054787
Sci Transl Med. 2020 Apr 22;12(540):
pubmed: 32321862
Nature. 2020 Sep;585(7826):584-587
pubmed: 32698191
Cell Rep. 2021 Oct 5;37(1):109773
pubmed: 34587479
Zool Res. 2019 Mar 18;40(2):89-93
pubmed: 30127329
JAMA Pediatr. 2020 Sep 01;174(9):882-889
pubmed: 32320004
Radiology. 2020 Jun;295(3):715-721
pubmed: 32053470
JAMA. 2020 Apr 7;323(13):1239-1242
pubmed: 32091533
Lancet. 2003 Jul 26;362(9380):263-70
pubmed: 12892955
Virus Res. 2008 Apr;133(1):20-32
pubmed: 17499378
Allergol Int. 2017 Oct;66(4):515-522
pubmed: 28826938
Cell. 2021 Mar 4;184(5):1188-1200.e19
pubmed: 33577765
Lab Anim Res. 2019 Jul 24;35:7
pubmed: 32257895
BMC Bioinformatics. 2013 Apr 15;14:128
pubmed: 23586463
J Clin Invest. 2020 Dec 1;130(12):6366-6378
pubmed: 32991329
Acta Paediatr. 2020 Jun;109(6):1088-1095
pubmed: 32202343
Pediatr Infect Dis J. 2020 Sep;39(9):e226-e233
pubmed: 32453194
Virol Sin. 2018 Apr;33(2):201-204
pubmed: 29541941
Int J Lab Hematol. 2020 Dec;42(6):750-760
pubmed: 32786136
Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16097-102
pubmed: 24043771
PLoS One. 2011 Apr 20;6(4):e18558
pubmed: 21533129
MMWR Morb Mortal Wkly Rep. 2020 Apr 10;69(14):422-426
pubmed: 32271728
JCI Insight. 2019 Feb 21;4(4):
pubmed: 30830861
J Zoo Wildl Med. 2014 Jun;45(2):306-14
pubmed: 25000691
Nat Commun. 2021 May 11;12(1):2670
pubmed: 33976165
BMC Biotechnol. 2005 Aug 18;5:22
pubmed: 16109166
Front Immunol. 2018 Aug 02;9:1750
pubmed: 30116242
N Engl J Med. 2020 Jul 9;383(2):187-190
pubmed: 32356945
Expert Rev Vaccines. 2009 Aug;8(8):1109-21
pubmed: 19627191
Pediatrics. 2020 Jun;145(6):
pubmed: 32179660
Lancet Infect Dis. 2020 Apr;20(4):425-434
pubmed: 32105637
Clin Infect Dis. 2021 Dec 6;73(11):e4154-e4165
pubmed: 33388749
Nat Biotechnol. 2022 Mar;40(3):319-324
pubmed: 34408314
JCI Insight. 2021 May 10;6(9):
pubmed: 33822777
Helicobacter. 2011 Sep;16 Suppl 1:70-5
pubmed: 21896089
Vaccines (Basel). 2021 Jun 01;9(6):
pubmed: 34206053
Front Cell Infect Microbiol. 2020 Dec 09;10:575559
pubmed: 33363049
J Clin Invest. 2020 Dec 1;130(12):6194-6197
pubmed: 33108354
Virology. 2008 Nov 10;381(1):89-97
pubmed: 18801550
Curr Protoc. 2021 Mar;1(3):e90
pubmed: 33780170
J Clin Immunol. 2021 Oct;41(7):1446-1456
pubmed: 34448086
Crit Rev Clin Lab Sci. 2017 Sep;54(6):358-413
pubmed: 29017389
Gut Microbes. 2021 Jan-Dec;13(1):1-19
pubmed: 33685349
Cell Rep. 2021 Mar 16;34(11):108852
pubmed: 33730580
Annu Int Conf IEEE Eng Med Biol Soc. 2014;2014:5667-70
pubmed: 25571281
Nat Immunol. 2022 Jan;23(1):40-49
pubmed: 34937928
Pathog Immun. 2019 Jan 29;4(1):1-20
pubmed: 30993250
JAMA. 2020 Apr 14;323(14):1335
pubmed: 32181795
Lancet Infect Dis. 2020 Jun;20(6):689-696
pubmed: 32220650
Front Pediatr. 2020 Jul 16;8:388
pubmed: 32766187
Front Pharmacol. 2019 Apr 26;10:445
pubmed: 31133849
J Immunol. 2018 May 1;200(9):3117-3127
pubmed: 29581354
Lancet. 2020 Jun 6;395(10239):1771-1778
pubmed: 32410760
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613
pubmed: 30476243
Nature. 2003 May 15;423(6937):240
pubmed: 12748632
PeerJ. 2016 Oct 18;4:e2584
pubmed: 27781170
Sci Transl Med. 2020 Oct 7;12(564):
pubmed: 32958614
PLoS One. 2009;4(2):e4505
pubmed: 19229336
Nature. 2021 Sep;597(7875):166-168
pubmed: 34493845
Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7
pubmed: 27141961
Cell. 2021 Apr 1;184(7):1858-1864.e10
pubmed: 33631096
Nat Methods. 2017 Apr;14(4):417-419
pubmed: 28263959
Bioinformatics. 2018 Apr 15;34(8):1287-1294
pubmed: 29228191
Bioinformatics. 2018 Sep 15;34(18):3094-3100
pubmed: 29750242
PLoS Med. 2006 May;3(5):e194
pubmed: 16608385