Advances towards Cell-Specific Gene Transfection: A Small-Molecule Approach Allows Order-of-Magnitude Selectivity.
biotin
cancer cell targeting
cell-penetrating peptides
gene technology
small molecules
Journal
Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783
Informations de publication
Date de publication:
01 Aug 2022
01 Aug 2022
Historique:
received:
30
12
2021
pubmed:
24
5
2022
medline:
5
8
2022
entrez:
23
5
2022
Statut:
ppublish
Résumé
A transfection vector that can home in on tumors is reported. Whereas previous vectors that allow moderately cell selective gene transfection used larger systems, this small-molecule approach paved the way for precise structure-activity relationship optimization. For this, biotin, which mediates cell selectivity, was combined with the potent DNA-binding motif tetralysine-guanidinocarbonypyrrol via a hydrophilic linker, thus enabling SAR-based optimization. The new vector mediated biotin receptor (BR)-selective transfection of cell lines with different BR expression levels. Computer-based analyses of microscopy images revealed a preference of one order of magnitude for the BR-positive cell lines over the BR-negative controls.
Identifiants
pubmed: 35604769
doi: 10.1002/chem.202104618
pmc: PMC9401007
doi:
Substances chimiques
Biotin
6SO6U10H04
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202104618Subventions
Organisme : Deutsche Forschungsgemeinschaft
Organisme : Evonik Stiftung
Informations de copyright
© 2022 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.
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