Identification, expression and subcellular localization of Orc1 in the microsporidian Nosema bombycis.

Animals Bombyx / genetics Nosema / genetics Origin Recognition Complex / genetics
Microsporidia Nosema bombycis Origin recognition complex SAS-6

Journal

Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761

Informations de publication

Date de publication:
05 Aug 2022
Historique:
received: 13 04 2022
revised: 14 05 2022
accepted: 18 05 2022
pubmed: 25 5 2022
medline: 22 6 2022
entrez: 24 5 2022
Statut: ppublish

Résumé

As a typical species of microsporidium, Nosema bombycis is the pathogen causing the pébrine disease of silkworm. Rapid proliferation of N. bombycis in host cells requires replication of genetic material. As eukaryotic origin recognition protein, origin recognition complex (ORC) plays an important role in regulating DNA replication, and Orc1 is a key subunit of the origin recognition complex. In this study, we identified the Orc1 in the microsporidian N. bombycis (NbOrc1) for the first time. The NbOrc1 gene contains a complete ORF of 987 bp in length that encodes a 328 amino acid polypeptide. Indirect immunofluorescence results showed that NbOrc1 were colocalized with Nbactin and NbSAS-6 in the nuclei of N. bombycis. Subsequently, we further identified the interaction between the NbOrc1 and Nbactin by CO-IP and Western blot. These results imply that Orc1 may be involved in the proliferation of the microsporidian N. bombycis through interacting with actin.

Identifiants

DOI: 10.1016/j.gene.2022.146607 PMID: 35609797
pubmed: 35609797
pii: S0378-1119(22)00426-7
doi: 10.1016/j.gene.2022.146607
pii:
doi:

Substances chimiques

Origin Recognition Complex 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

146607

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Fuzhen Sun (F)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Guanyu Zhu (G)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Ping He (P)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Erjun Wei (E)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Runpeng Wang (R)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Qiang Wang (Q)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China; Sericulture Research Institute of Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China.

Xudong Tang (X)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China; Sericulture Research Institute of Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China.

Yiling Zhang (Y)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China; Sericulture Research Institute of Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China.

Zhongyuan Shen (Z)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China; Sericulture Research Institute of Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China. Electronic address: szysri@163.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Identification, expression and subcellular...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Lepidoptera › Papillons de nuit › Bombyx › Identification, expression and subcellular...
questionsmedicales.fr › Eucaryotes › Champignons › Microsporidia › Microsporea › Microsporida › Apansporoblastina › Nosema › Identification, expression and subcellular...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Complexe ORC › Identification, expression and subcellular...