Comparative genomic analysis of ESBL-producing
Journal
JAC-antimicrobial resistance
ISSN: 2632-1823
Titre abrégé: JAC Antimicrob Resist
Pays: England
ID NLM: 101765283
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
15
11
2021
accepted:
02
05
2022
entrez:
25
5
2022
pubmed:
26
5
2022
medline:
26
5
2022
Statut:
epublish
Résumé
The reliability of ESBL-producing To compare the genomic characteristics of ESBL-Ec isolates from faecal carriage and FUTI to assess their correlation and identify a FUTI-associated virulence profile. We conducted a prospective multicentre hospital and ambulatory-based study. We analysed the genotypes and virulence factors of both faecal and FUTI ESBL-Ec by whole genome sequencing. Correlations were assessed by non-parametric Spearman coefficient and virulence factors were assessed by chi-squared tests with Bonferroni correction. We included 218 ESBL-Ec causing FUTI and 154 ESBL-Ec faecal carriage isolates. The most frequent ST was ST131 (44%) in both collections. We found high correlation between carriage and ESBL-Ec FUTI regarding genes/alleles (rho = 0.88, The genomic profile of ESBL-Ec causing FUTI in children strongly correlates with faecal carriage isolates except for a few genes. The presence of
Sections du résumé
Background
UNASSIGNED
The reliability of ESBL-producing
Objectives
UNASSIGNED
To compare the genomic characteristics of ESBL-Ec isolates from faecal carriage and FUTI to assess their correlation and identify a FUTI-associated virulence profile.
Methods
UNASSIGNED
We conducted a prospective multicentre hospital and ambulatory-based study. We analysed the genotypes and virulence factors of both faecal and FUTI ESBL-Ec by whole genome sequencing. Correlations were assessed by non-parametric Spearman coefficient and virulence factors were assessed by chi-squared tests with Bonferroni correction.
Results
UNASSIGNED
We included 218 ESBL-Ec causing FUTI and 154 ESBL-Ec faecal carriage isolates. The most frequent ST was ST131 (44%) in both collections. We found high correlation between carriage and ESBL-Ec FUTI regarding genes/alleles (rho = 0.88,
Conclusions
UNASSIGNED
The genomic profile of ESBL-Ec causing FUTI in children strongly correlates with faecal carriage isolates except for a few genes. The presence of
Identifiants
pubmed: 35611261
doi: 10.1093/jacamr/dlac056
pii: dlac056
pmc: PMC9123598
doi:
Types de publication
Journal Article
Langues
eng
Pagination
dlac056Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.
Références
Infect Immun. 2005 Jul;73(7):4081-7
pubmed: 15972496
PLoS One. 2018 Jan 25;13(1):e0190910
pubmed: 29370234
Infect Dis (Lond). 2016 Sep;48(9):695-8
pubmed: 27225534
Microb Genom. 2018 Jul;4(7):
pubmed: 29916797
J Antimicrob Chemother. 2016 Oct;71(10):2949-56
pubmed: 27330068
Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5694-9
pubmed: 24706808
Clin Microbiol Rev. 2005 Apr;18(2):417-22
pubmed: 15831830
Int J Med Microbiol. 2010 Nov;300(7):435-9
pubmed: 20510652
Microbiology (Reading). 2016 Apr;162(4):642-650
pubmed: 26822436
J Clin Microbiol. 2012 Apr;50(4):1355-61
pubmed: 22238442
Clin Microbiol Rev. 2014 Jul;27(3):543-74
pubmed: 24982321
Front Microbiol. 2019 Jul 16;10:1530
pubmed: 31379759
J Antimicrob Chemother. 2021 Mar 12;76(4):901-908
pubmed: 33428729
Infect Immun. 1998 Sep;66(9):4411-7
pubmed: 9712795
Antimicrob Agents Chemother. 2019 Jun 24;63(7):
pubmed: 31085515
J Antimicrob Chemother. 2020 Jan 1;75(1):96-105
pubmed: 31617912
mBio. 2016 Apr 26;7(2):e00347-16
pubmed: 27118589
Lancet Infect Dis. 2008 Mar;8(3):159-66
pubmed: 18291338
J Clin Microbiol. 2005 Jun;43(6):2962-4
pubmed: 15956432
Infect Immun. 2008 Jul;76(7):2978-90
pubmed: 18443089
J Med Microbiol. 2001 Jul;50(7):650-652
pubmed: 11444777
J Antimicrob Chemother. 2008 Feb;61(2):273-81
pubmed: 18077311
J Clin Microbiol. 2003 Jan;41(1):285-9
pubmed: 12517862
J Clin Microbiol. 2015 Aug;53(8):2410-26
pubmed: 25972421
J Infect. 2016 Dec;73(6):547-557
pubmed: 27475789
J Clin Microbiol. 2017 Aug;55(8):2538-2543
pubmed: 28592545
J Antimicrob Chemother. 2020 Dec 1;75(12):3491-3500
pubmed: 32780112
Clin Infect Dis. 2007 Oct 1;45(7):846-52
pubmed: 17806048
J Bacteriol. 2007 May;189(9):3532-46
pubmed: 17351047
J Antimicrob Chemother. 2017 Sep 1;72(9):2410-2417
pubmed: 28637307
Infect Immun. 2008 Mar;76(3):1135-42
pubmed: 18160475
BMJ. 1989 Sep 16;299(6701):703-6
pubmed: 2508881
Infect Immun. 2009 Jun;77(6):2272-84
pubmed: 19307211
Pediatr Nephrol. 2016 Aug;31(8):1253-65
pubmed: 26238274
J Urol. 1997 Oct;158(4):1610-3
pubmed: 9302184
J Infect Dis. 2004 Nov 15;190(10):1739-44
pubmed: 15499527
Pathogens. 2015 Jun 26;4(3):422-30
pubmed: 26131613
Infect Immun. 2004 Jan;72(1):478-88
pubmed: 14688129
JAMA Pediatr. 2016 Sep 1;170(9):848-54
pubmed: 27455161