Characterization of a Bacillus cereus strain associated with a large feed-related outbreak of severe infection in pigs.
Animal Feed
/ microbiology
Animals
Bacillus cereus
/ genetics
Disease Outbreaks
Gram-Positive Bacteria
Gram-Positive Bacterial Infections
/ microbiology
Hemolysin Proteins
/ genetics
Larva
/ microbiology
Moths
/ microbiology
Peptide Hydrolases
Random Amplified Polymorphic DNA Technique
Spores, Bacterial
Swine
Bacillus cereus
outbreak
pathogenicity
strain characterization
swine
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
revised:
12
05
2022
received:
03
02
2022
accepted:
19
05
2022
pubmed:
26
5
2022
medline:
10
8
2022
entrez:
25
5
2022
Statut:
ppublish
Résumé
Bacillus cereus is often responsible for foodborne diseases and both local and systemic infections in humans. Cases of infection in other mammals are rather rare. In this study, we report a B. cereus feed-related outbreak that caused the death of 6234 pigs in Italy. Massive doses of a Gram-positive, spore-forming bacterium were recovered from the animal feed, faeces of survived pigs and intestinal content of dead ones. The B. cereus MM1 strain was identified by MALDI-TOF MS and typified by RAPD-PCR. The isolate was tested for the production of PC-PLC, proteases, hemolysins and biofilm, for motility, as well as for the presence of genes encoding tissue-degrading enzymes and toxins. Antimicrobial resistance and pathogenicity in Galleria mellonella larvae were also investigated. Our results show that the isolated B. cereus strain is swimming-proficient, produces PC-PLC, proteases, hemolysins, biofilm and carries many virulence genes. The strain shows high pathogenicity in G. mellonella larvae. The isolated B. cereus strain demonstrates an aggressive profile of pathogenicity and virulence, being able to produce a wide range of determinants potentially hazardous to pigs' health. This study highlights the proficiency of B. cereus to behave as a devastating pathogen in swine if ingested at high doses and underlines that more stringent quality controls are needed for livestock feeds and supplements.
Identifiants
pubmed: 35611609
doi: 10.1111/jam.15636
pmc: PMC9543730
doi:
Substances chimiques
Hemolysin Proteins
0
Peptide Hydrolases
EC 3.4.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1078-1088Informations de copyright
© 2022 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.
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