Calcium-dependent transcriptional changes in human pancreatic islet cells reveal functional diversity in islet cell subtypes.
Calcium signalling
Islet heterogeneity
Islets of Langerhans
Polyhormonal cells
Single-cell RNA sequencing
Transcriptional regulation
Journal
Diabetologia
ISSN: 1432-0428
Titre abrégé: Diabetologia
Pays: Germany
ID NLM: 0006777
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
received:
06
08
2021
accepted:
11
02
2022
pubmed:
27
5
2022
medline:
5
8
2022
entrez:
26
5
2022
Statut:
ppublish
Résumé
Pancreatic islets depend on cytosolic calcium (Ca We exposed intact human islets from three donors to the following conditions: (1) 2.8 mmol/l glucose; (2) 16 mmol/l glucose and 40 mmol/l KCl to maximally stimulate Ca Based on the number of Ca Here we use our large-scale, multi-condition, single-cell dataset to show that human islets have cell-type-specific Ca A searchable and user-friendly format of the data in this study, specifically designed for rapid mining of single-cell RNA sequencing data, is available at https://lynnlab.shinyapps.io/Human_Islet_Atlas/ . The raw data files are available at NCBI Gene Expression Omnibus (GSE196715).
Identifiants
pubmed: 35616696
doi: 10.1007/s00125-022-05718-1
pii: 10.1007/s00125-022-05718-1
pmc: PMC9345846
doi:
Substances chimiques
Insulin
0
Glucose
IY9XDZ35W2
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Retracted Publication
Langues
eng
Sous-ensembles de citation
IM
Pagination
1519-1533Subventions
Organisme : CIHR
ID : MOP 142222
Pays : Canada
Commentaires et corrections
Type : RetractionIn
Informations de copyright
© 2022. The Author(s).
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