A New Transgenic Line for Rapid and Complete Neutrophil Ablation.
NTR 2.0
ablation
metronidazole
neutrophil
nitroreductase
Journal
Zebrafish
ISSN: 1557-8542
Titre abrégé: Zebrafish
Pays: United States
ID NLM: 101225070
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
pubmed:
27
5
2022
medline:
18
6
2022
entrez:
26
5
2022
Statut:
ppublish
Résumé
Zebrafish lines expressing nitroreductase (NTR) in specific cell compartments, which sensitizes those cells to metronidazole (MTZ)-mediated ablation, have proven extremely useful for studying tissue regeneration and investigating cell function. In contrast to many cells, neutrophils are comparatively resistant to the NTR/MTZ targeted ablation strategy. Recently, a rationally engineered variant of NTR (NTR 2.0) has been described that exhibits greatly improved MTZ-mediated ablation efficacy in zebrafish. We show that a transgenic line with neutrophil-restricted expression of NTR 2.0 demonstrates complete neutrophil ablation, with an MTZ dose 100-fold less than current treatment regimens, and with treatment durations as short as 5 h.
Identifiants
pubmed: 35617702
doi: 10.1089/zeb.2022.0020
pmc: PMC9246267
doi:
Substances chimiques
Metronidazole
140QMO216E
Nitroreductases
EC 1.7.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
109-113Subventions
Organisme : NIH HHS
ID : R01 OD020376
Pays : United States
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