Transcriptome analysis of CRISPR/Cas9-mediated GPAM
Animals
CRISPR-Cas Systems
Cattle
Epithelial Cells
/ metabolism
Fatty Acids
/ metabolism
Fatty Acids, Unsaturated
/ metabolism
Gene Expression Profiling
Glycerol-3-Phosphate O-Acyltransferase
/ genetics
Lipid Metabolism
/ genetics
Mammary Glands, Animal
/ metabolism
Milk
/ metabolism
Phosphates
/ metabolism
BMECs
CRISPR/Cas9
GPAM
Lipid metabolism
Pathways
Journal
Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761
Informations de publication
Date de publication:
05 Aug 2022
05 Aug 2022
Historique:
received:
21
11
2021
revised:
05
05
2022
accepted:
09
05
2022
pubmed:
27
5
2022
medline:
22
6
2022
entrez:
26
5
2022
Statut:
ppublish
Résumé
Glycerol-3-phosphate acyltransferase mitochondrial (GPAM) is an enzyme in animal lipid metabolism pathways that catalyzes the initial and most committed step of glycerolipid biosynthesis. The present study mainly focused on exploring the relationship between the GPAM gene and the lipid metabolism of mammary epithelial cells and the effect of GPAM on the related pathways of lipid metabolism. The GPAM gene was knocked out entirely in bovine mammary epithelial cells(BMECs) using CRISPR/Cas9 technology, and the mechanism by which the GPAM gene regulates lipid metabolism in BMECs was confirmed. Furthermore, after the complete loss of GPAM, BMECs' triglycerides (TGs) and cholesterol (CHOL) levels were significantly decreased (p < 0.05). Concurrently, the content of octanoic acid, a medium-chain saturated fatty acid, increased substantially in BMECs. RNA-seq of GPAM
Identifiants
pubmed: 35618221
pii: S0378-1119(22)00393-6
doi: 10.1016/j.gene.2022.146574
pii:
doi:
Substances chimiques
Fatty Acids
0
Fatty Acids, Unsaturated
0
Phosphates
0
Glycerol-3-Phosphate O-Acyltransferase
EC 2.3.1.15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
146574Informations de copyright
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