A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component.


Journal

The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664

Informations de publication

Date de publication:
04 07 2022
Historique:
revised: 15 04 2022
received: 03 12 2021
accepted: 21 04 2022
pubmed: 28 5 2022
medline: 8 7 2022
entrez: 27 5 2022
Statut: ppublish

Résumé

Beyond its role in cellular homeostasis, autophagy plays anti- and promicrobial roles in host-microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well-described in animals, the extent to which xenophagy contributes to plant-bacteria interactions remains unknown. Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type-III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. Intriguingly, XopL is targeted for degradation by defense-related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense-related autophagy in plant-bacteria interactions.

Identifiants

pubmed: 35620914
doi: 10.15252/embj.2021110352
pmc: PMC9251887
doi:

Substances chimiques

Virulence Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e110352

Informations de copyright

© 2022 The Authors. Published under the terms of the CC BY 4.0 license.

Références

Science. 2019 Nov 15;366(6467):818-822
pubmed: 31727826
Cell. 2003 Oct 31;115(3):333-42
pubmed: 14636560
Elife. 2021 Aug 23;10:
pubmed: 34424198
Mol Plant Microbe Interact. 2019 Sep;32(9):1229-1242
pubmed: 31012804
J Biol Chem. 2005 Nov 18;280(46):38682-8
pubmed: 16176924
Plant Physiol. 2016 Nov;172(3):1941-1958
pubmed: 27613851
Plant Physiol. 2018 Jan;176(1):649-662
pubmed: 29133371
Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):E7197-E7204
pubmed: 28784794
Plant Cell. 2017 Jun;29(6):1388-1405
pubmed: 28584166
J Exp Bot. 2018 Aug 31;69(19):4529-4537
pubmed: 29873762
Cell Res. 2016 Apr;26(4):457-83
pubmed: 27012466
Trends Microbiol. 2020 Jul;28(7):523-535
pubmed: 32544439
Mol Cell. 2014 Apr 24;54(2):224-33
pubmed: 24766886
Plant J. 1998 Dec;16(6):735-43
pubmed: 10069079
Plant Cell. 2013 Nov;25(11):4596-615
pubmed: 24249832
Plant Cell. 2017 Apr;29(4):726-745
pubmed: 28280093
EMBO J. 2022 Jul 4;41(13):e110352
pubmed: 35620914
Front Plant Sci. 2016 Nov 30;7:1796
pubmed: 27965697
Genome Res. 2010 Jun;20(6):837-46
pubmed: 20237107
J Immunol. 2009 Nov 1;183(9):5909-16
pubmed: 19812211
Plant Cell. 2018 Mar;30(3):668-685
pubmed: 29500318
Mol Plant Microbe Interact. 2014 Jul;27(7):611-23
pubmed: 24625030
PLoS Pathog. 2012;8(6):e1002743
pubmed: 22719249
Cell Host Microbe. 2009 Aug 20;6(2):137-49
pubmed: 19683680
Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):E2026-E2035
pubmed: 28223514
Elife. 2018 Jun 22;7:
pubmed: 29932422
Mol Plant. 2017 Aug 7;10(8):1026-1034
pubmed: 28698057
Plant J. 2018 Feb;93(4):651-663
pubmed: 29160935
Cell Microbiol. 2015 Jan;17(1):18-25
pubmed: 25339602
J Bacteriol. 2009 Mar;191(5):1414-28
pubmed: 19114489
Autophagy. 2014 Apr;10(4):704-5
pubmed: 24598432
Mol Cell. 2012 Sep 14;47(5):797-809
pubmed: 22819327
Autophagy. 2011 Sep;7(9):993-1010
pubmed: 21606687
PLoS Pathog. 2013;9(6):e1003427
pubmed: 23785289
Plant Physiol. 2019 Nov;181(3):855-866
pubmed: 31488572
Front Plant Sci. 2014 Dec 18;5:736
pubmed: 25566304
PLoS Genet. 2013;9(1):e1003196
pubmed: 23341779
Cell Host Microbe. 2013 Feb 13;13(2):143-54
pubmed: 23414755
Curr Opin Plant Biol. 2019 Dec;52:46-53
pubmed: 31442734
Cell Death Differ. 2020 Mar;27(3):858-871
pubmed: 31900427
J Exp Bot. 2018 Mar 14;69(6):1415-1432
pubmed: 29365132
FEMS Microbiol Rev. 2016 Nov 1;40(6):894-937
pubmed: 28201715
Cell Microbiol. 2013 Mar;15(3):395-402
pubmed: 23121192
PLoS Pathog. 2013 Jan;9(1):e1003121
pubmed: 23359647
Plant Cell. 2008 Jul;20(7):1915-29
pubmed: 18664616
Plant J. 2018 Mar;93(5):856-870
pubmed: 29285819
Front Plant Sci. 2015 Aug 03;6:599
pubmed: 26284106
Biochem Biophys Res Commun. 2017 Dec 9;494(1-2):20-26
pubmed: 29056507
Mol Plant. 2020 Oct 5;13(10):1513-1522
pubmed: 32889173
Nat Rev Microbiol. 2014 Feb;12(2):101-14
pubmed: 24384599
Autophagy. 2009 Jul;5(5):732-3
pubmed: 19398892
Nat Commun. 2019 Apr 29;10(1):1973
pubmed: 31036822
Plant J. 2011 Jun;66(5):818-30
pubmed: 21332848
Cell Death Differ. 2019 Mar;26(4):703-714
pubmed: 30737478
Autophagy. 2018;14(8):1465-1466
pubmed: 30033807
J Biosci Bioeng. 2007 Jul;104(1):34-41
pubmed: 17697981
Nature. 2011 Jan 20;469(7330):323-35
pubmed: 21248839
Front Plant Sci. 2012 Oct 24;3:238
pubmed: 23109936
Curr Opin Plant Biol. 2017 Dec;40:122-130
pubmed: 28946008
Gene. 1995 Dec 1;166(1):175-6
pubmed: 8529885
Plant Physiol. 2015 May;168(1):107-19
pubmed: 25739698
Front Cell Infect Microbiol. 2019 Jul 31;9:270
pubmed: 31428589
Nat Rev Microbiol. 2020 Aug;18(8):415-427
pubmed: 32346148
Plant J. 2017 Dec;92(5):787-795
pubmed: 28891100
Cell Host Microbe. 2020 Oct 7;28(4):558-571.e6
pubmed: 32810441
Plant J. 2010 Oct;64(2):355-65
pubmed: 20735773
Elife. 2016 Jan 14;5:
pubmed: 26765567
Nature. 2011 May 19;473(7347):337-42
pubmed: 21593866
Plant Cell. 2019 Oct;31(10):2456-2474
pubmed: 31266900
Nat Methods. 2017 May;14(5):504-512
pubmed: 28319114
Annu Rev Plant Biol. 2018 Apr 29;69:173-208
pubmed: 29539270
Plant Mol Biol. 2018 Jun;97(3):201-214
pubmed: 29679263

Auteurs

Jia Xuan Leong (JX)

Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany.

Margot Raffeiner (M)

Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), Großbeeren, Germany.

Daniela Spinti (D)

Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), Großbeeren, Germany.

Gautier Langin (G)

Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany.

Mirita Franz-Wachtel (M)

Interfaculty Institute for Cell Biology, Department of Quantitative Proteomics, University of Tübingen, Tübingen, Germany.

Andrew R Guzman (AR)

Department of Biology, Stanford University, Stanford, CA, USA.

Jung-Gun Kim (JG)

Department of Biology, Stanford University, Stanford, CA, USA.

Pooja Pandey (P)

Department of Life Sciences, Imperial College London, London, UK.

Alyona E Minina (AE)

Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden.

Boris Macek (B)

Interfaculty Institute for Cell Biology, Department of Quantitative Proteomics, University of Tübingen, Tübingen, Germany.

Anders Hafrén (A)

Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden.

Tolga O Bozkurt (TO)

Department of Life Sciences, Imperial College London, London, UK.

Mary Beth Mudgett (MB)

Department of Biology, Stanford University, Stanford, CA, USA.

Frederik Börnke (F)

Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), Großbeeren, Germany.
Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany.

Daniel Hofius (D)

Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden.

Suayib Üstün (S)

Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany.
Faculty of Biology & Biotechnology, Ruhr-University Bochum, Bochum, Germany.

Articles similaires

Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH