The role of ephrinB2-EphB4 signalling in bone remodelling during orthodontic tooth movement.
EphB4
alveolar bone remodelling
ephrinB2
orthodontic tooth movement
Journal
Orthodontics & craniofacial research
ISSN: 1601-6343
Titre abrégé: Orthod Craniofac Res
Pays: England
ID NLM: 101144387
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
revised:
15
03
2022
received:
16
07
2021
accepted:
16
05
2022
pubmed:
28
5
2022
medline:
7
1
2023
entrez:
27
5
2022
Statut:
ppublish
Résumé
The aim of this study was to investigate the role of ephrinB2-EphB4 signalling in alveolar bone remodelling on the tension side during orthodontic tooth movement (OTM). An OTM model was established on sixty 8-week-old male Wistar rats. They were randomly divided into the experimental group and the control group. The animals in the experimental group were administrated with subcutaneous injection of EphB4 inhibitor NVP-BHG712 every other day, whereas the control group received only the vehicle. Samples containing the maxillary first molar and the surrounding bone were collected after 0, 3, 7, 14 and 21 days of tooth movement. EphrinB2-EphB4 signalling was actively expressed on the tension side during tooth movement. Micro-CT analysis showed the distance of tooth movement in the experimental group was significantly greater than that of the control group (P < .05) with significantly increased trabecular separation (Tb. Sp) and decreased trabecular number (Tb. N) from day 14 to day 21. The number of osteoclasts significantly increased in the experimental group compared with the control group after 3 and 7 days of tooth movement (P < .05). The expressions of alkaline phosphatase (ALP) and osteopontin (OPN) were significantly reduced by inhibition of EphB4 (P < .05). The inhibition of EphB4 suppressed bone formation and enhanced bone resorption activities on the tension side of tooth movement. The ephrinB2-EphB4 signalling might play an important role in alveolar bone remodelling during OTM.
Substances chimiques
Ephrin-B2
0
Ephrins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107-116Subventions
Organisme : National Natural Science Foundation of China
Organisme : Research Funding from West China School/Hospital of Stomatology, Sichuan University
Organisme : Sichuan University Postdoctoral Interdisciplinary Innovation Fund
Informations de copyright
© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Références
Guram G, Reddy RK, Dharamsi AM, Syed Ismail PM, Mishra S, Prakashkumar MD. Evaluation of low-level laser therapy on orthodontic tooth movement: a randomized control study. Contemp Clin Dent. 2018;9(1):105-109.
Huang H, Yang R, Zhou YH. Mechanobiology of periodontal ligament stem cells in orthodontic tooth movement. Stem Cells Int. 2018;2018:6531216.
Batra N, Kar R, Jiang JX. Gap junctions and hemichannels in signal transmission, function and development of bone. Biochim Biophys Acta. 2012;1818(8):1909-1918.
Jin H, Yao L, Chen K, et al. Evodiamine inhibits RANKL-induced osteoclastogenesis and prevents ovariectomy-induced bone loss in mice. J Cell Mol Med. 2019;23(1):522-534.
Wise GE, King GJ. Mechanisms of tooth eruption and orthodontic tooth movement. J Dent Res. 2008;87(5):414-434.
Hemanth M, Deoli S, Raghuveer HP, Rani MS, Hegde C, Vedavathi B. Stress induced in periodontal ligament under orthodontic loading (part II): a comparison of linear versus non-linear fem study. J Int Oral Health. 2015;7(9):114-118.
Omidkhoda M, Radvar M, Azizi M, Hasanzadeh N. Piezopuncture-assisted canine distalization in orthodontic patients: two case reports. J Dent. 2018;19(1):74-82.
An J, Li Y, Liu Z, Wang R, Zhang B. A micro-CT study of microstructure change of alveolar bone during orthodontic tooth movement under different force magnitudes in rats. Exp Ther Med. 2017;13(5):1793-1798.
Unified nomenclature for Eph family receptors and their ligands, the ephrins. Eph nomenclature committee. Cell. 1997;90(3):403-404.
Himanen JP, Nikolov DB. Eph receptors and ephrins. Int J Biochem Cell Biol. 2003;35(2):130-134.
Kullander K, Klein R. Mechanisms and functions of Eph and ephrin signalling. Nat Rev Mol Cell Biol. 2002;3(7):475-486.
Muñoz JJ, Cejalvo T, Alonso-Colmenar LM, Alfaro D, Garcia-Ceca J, Zapata A. Eph/ephrin-mediated interactions in the thymus. Neuroimmunomodulation. 2011;18(5):271-280.
Hou J, Chen Y, Meng X, et al. Compressive force regulates ephrinB2 and EphB4 in osteoblasts and osteoclasts contributing to alveolar bone resorption during experimental tooth movement. Korean J Orthod. 2014;44(6):320-329.
Martin TJ, Allan EH, Ho PW, et al. Communication between ephrinB2 and EphB4 within the osteoblast lineage. Adv Exp Med Biol. 2010;658:51-60.
Matsuo K, Irie N. Osteoclast-osteoblast communication. Arch Biochem Biophys. 2008;473(2):201-209.
Diercke K, Kohl A, Lux CJ, Erber R. Strain-dependent up-regulation of ephrin-B2 protein in periodontal ligament fibroblasts contributes to osteogenesis during tooth movement. J Biol Chem. 2011;286(43):37651-37664.
Arifin WN, Zahiruddin WM. Sample size calculation in animal studies using resource equation approach. Malays J Med Sci. 2017;24(5):101-105.
Li F, Li G, Hu H, Liu R, Chen J, Zou S. Effect of parathyroid hormone on experimental tooth movement in rats. Am J Orthod Dentofac Orthop. 2013;144(4):523-532.
Jiang L, Tang Z. Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement. Mol Med Rep. 2018;17(1):1499-1506.
Taddei SR, Moura AP, Andrade I Jr, et al. Experimental model of tooth movement in mice: a standardized protocol for studying bone remodeling under compression and tensile strains. J Biomech. 2012;45(16):2729-2735.
Tsolakis AI, Khaldi L, Bitsanis I, et al. The effect of osteopenia on tooth movement in ovariectomized rats. An experimental study. J Musculoskelet Neuronal Interact. 2018;18(3):366-374.
Li T, Wang H, Lv C, et al. Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling. J Cell Physiol. 2021;236(3):2070-2086.
Pasquale EB. Eph receptor signalling casts a wide net on cell behaviour. Nat Rev Mol Cell Biol. 2005;6(6):462-475.
Obi S, Yamamoto K, Shimizu N, et al. Fluid shear stress induces arterial differentiation of endothelial progenitor cells. J Appl Physiol. 2009;106(1):203-211.
Zhao C, Irie N, Takada Y, et al. Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab. 2006;4(2):111-121.
Irie N, Takada Y, Watanabe Y, et al. Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis. J Biol Chem. 2009;284(21):14637-14644.
Alikhani M, Alyami B, Lee IS, et al. Saturation of the biological response to orthodontic forces and its effect on the rate of tooth movement. Orthod Craniofac Res. 2015;18(1):8-17.
Gudhimella S, Ibrahim AY, Karanth D, et al. A rodent model using skeletal anchorage and low forces for orthodontic tooth movement. Am J Orthod Dentofacial Orthop. 2019;155(2):254-263.
Guo Z, Kang S, Chen D, et al. MAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to bacillus thuringiensis Cry1Ac toxin in diamondback moth. PLoS Genet. 2015;11(4):e1005124.
Zhang Z, Zhang X, Zhao D, et al. TGF-β1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway. Mol Med Rep. 2019;19(5):3505-3518.
Zhang R, Zhang Z, Pan X, Huang X, Huang Z, Zhang G. ATX-LPA axis induces expression of OPN in hepatic cancer cell SMMC7721. Anat Rec. 2011;294(3):406-411.
De Luca I, Di Salle A, Alessio N, et al. Positively charged polymers modulate the fate of human mesenchymal stromal cells via ephrinB2/EphB4 signaling. Stem Cell Res. 2016;17(2):248-255.
Wang LM, Zhao N, Zhang J, Sun QF, Yang CZ, Yang PS. Tumor necrosis factor-alpha inhibits osteogenic differentiation of pre-osteoblasts by downregulation of EphB4 signaling via activated nuclear factor-kappaB signaling pathway. J Periodontal Res. 2018;53(1):66-72.
Suzuki SS, Garcez AS, Suzuki H, Ervolino E, Moon W, Ribeiro MS. Low-level laser therapy stimulates bone metabolism and inhibits root resorption during tooth movement in a rodent model. J Biophotonics. 2016;9(11-12):1222-1235.