Impact of Different Cell Counting Methods in Molecular Monitoring of Chronic Myeloid Leukemia Patients.
BCR-ABL1/ABL1
Q-PCR
cell count
chronic myeloid leukemia
Journal
Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402
Informations de publication
Date de publication:
22 Apr 2022
22 Apr 2022
Historique:
received:
02
04
2022
revised:
14
04
2022
accepted:
21
04
2022
entrez:
28
5
2022
pubmed:
29
5
2022
medline:
29
5
2022
Statut:
epublish
Résumé
Detection of We tested five different types of measurements: one manual hemocytometer-based count and four FC-based automatic cell-counting methods, including absolute, based on beads, based on 7-amino actinomycin D, combining and associating beads and 7AAD. The recovery efficiency for each counting method was established considering the quality and quantity of total RNA isolated and the Q-PCR results in matched samples from 90 adults with CML. Our analyses showed no consistent bias between the different types of measurements, with comparable number of WBCs counted for each type of measurement. Similarly, we observed a 100% concordance in the amount of RNA extracted and in the Q-PCR cycle threshold values for both This automatic method can replace the more laborious manual workflow, especially when high-throughput isolations from blood of CML patients are needed.
Sections du résumé
BACKGROUND
BACKGROUND
Detection of
METHODS
METHODS
We tested five different types of measurements: one manual hemocytometer-based count and four FC-based automatic cell-counting methods, including absolute, based on beads, based on 7-amino actinomycin D, combining and associating beads and 7AAD. The recovery efficiency for each counting method was established considering the quality and quantity of total RNA isolated and the Q-PCR results in matched samples from 90 adults with CML.
RESULTS
RESULTS
Our analyses showed no consistent bias between the different types of measurements, with comparable number of WBCs counted for each type of measurement. Similarly, we observed a 100% concordance in the amount of RNA extracted and in the Q-PCR cycle threshold values for both
CONCLUSIONS
CONCLUSIONS
This automatic method can replace the more laborious manual workflow, especially when high-throughput isolations from blood of CML patients are needed.
Identifiants
pubmed: 35626209
pii: diagnostics12051051
doi: 10.3390/diagnostics12051051
pmc: PMC9140187
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
Leukemia. 2017 Apr;31(4):1015-1016
pubmed: 28093565
Hematology Am Soc Hematol Educ Program. 2015;2015:257-63
pubmed: 26637731
Chemotherapy. 2018;63(4):220-224
pubmed: 30372691
J Cancer Res Clin Oncol. 2021 Oct;147(10):3081-3089
pubmed: 33677711
Front Pharmacol. 2021 Jun 30;12:669469
pubmed: 34276365
Blood. 2009 Feb 19;113(8):1619-30
pubmed: 18827185
N Engl J Med. 2017 Mar 9;376(10):917-927
pubmed: 28273028
Clin Cancer Res. 2017 Dec 1;23(23):7189-7198
pubmed: 28928163
Blood. 2013 Aug 8;122(6):872-84
pubmed: 23803709
Int J Mol Sci. 2019 May 06;20(9):
pubmed: 31064152
Plant Methods. 2018 Apr 16;14:30
pubmed: 29686723
Blood. 2020 Feb 20;135(8):534-541
pubmed: 31877211
Blood. 2006 Jul 1;108(1):28-37
pubmed: 16522812
Br J Haematol. 2011 Apr;153(2):179-90
pubmed: 21382019
Int J Mol Sci. 2019 Dec 04;20(24):
pubmed: 31817063
Leukemia. 2012 Oct;26(10):2172-5
pubmed: 22504141
Leukemia. 2020 Apr;34(4):966-984
pubmed: 32127639
Ann Hematol. 2015 Apr;94 Suppl 2:S219-25
pubmed: 25814088
Leukemia. 2008 Jan;22(1):96-102
pubmed: 17943168
Am J Hematol. 2017 Jan;92(1):82-87
pubmed: 27770583
Nat Rev Cancer. 2005 Mar;5(3):172-83
pubmed: 15719031
Ann Hematol. 2021 Jul;100(7):1743-1753
pubmed: 33774681
Biotechnol Rep (Amst). 2015 Apr 18;7:9-16
pubmed: 28626709
Front Oncol. 2019 Sep 06;9:833
pubmed: 31555590
Blood. 2014 Feb 27;123(9):1353-60
pubmed: 24335106
Diagnostics (Basel). 2021 Aug 20;11(8):
pubmed: 34441436
Biologicals. 2010 May;38(3):393-400
pubmed: 20185335
PLoS One. 2015 Oct 16;10(10):e0140585
pubmed: 26473951
Leukemia. 2015 May;29(5):999-1003
pubmed: 25652737
Blood. 2018 Nov 15;132(20):2125-2133
pubmed: 30429156
Am J Hematol. 2020 Jun;95(6):691-709
pubmed: 32239758
Front Oncol. 2019 Aug 13;9:764
pubmed: 31456947
Lancet. 2015 Apr 11;385(9976):1447-59
pubmed: 25484026
Nat Rev Clin Oncol. 2017 Mar;14(3):141-154
pubmed: 27752053
J Clin Med. 2021 Nov 29;10(23):
pubmed: 34884309
J Reprod Fertil. 1964 Oct;8:149-55
pubmed: 14209117
Leukemia. 2016 Aug;30(8):1638-47
pubmed: 27133824
Anticancer Res. 2020 May;40(5):2457-2465
pubmed: 32366389
Best Pract Res Clin Haematol. 2016 Sep;29(3):308-313
pubmed: 27839571