Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture.
apoptosis
colon cancer cells
cytochrome c
differentiation
electron microscopy
inhibition of proliferation
plant polysaccharides
wheat cell culture
Journal
Pharmaceutics
ISSN: 1999-4923
Titre abrégé: Pharmaceutics
Pays: Switzerland
ID NLM: 101534003
Informations de publication
Date de publication:
20 May 2022
20 May 2022
Historique:
received:
05
04
2022
revised:
13
05
2022
accepted:
17
05
2022
entrez:
28
5
2022
pubmed:
29
5
2022
medline:
29
5
2022
Statut:
epublish
Résumé
There is a global need to discover effective anti-cancerous compounds from natural sources. Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides. In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase and induced different rates of apoptosis (≤48%). Transmission and scanning electron microscopy revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the anti-cancer capacity of WCCPS fractions in colorectal cancer.
Identifiants
pubmed: 35631686
pii: pharmaceutics14051100
doi: 10.3390/pharmaceutics14051100
pmc: PMC9147229
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
Sci Rep. 2018 Apr 18;8(1):6172
pubmed: 29670154
Plants (Basel). 2022 Jan 24;11(3):
pubmed: 35161284
Ann Surg Oncol. 2007 Dec;14(12):3453-9
pubmed: 17882496
Molecules. 2011 Oct 26;16(11):9003-16
pubmed: 22031064
CA Cancer J Clin. 2021 May;71(3):209-249
pubmed: 33538338
Cell Death Dis. 2019 Jun 11;10(6):456
pubmed: 31186406
J Clin Endocrinol Metab. 2002 Jul;87(7):3475-85
pubmed: 12107268
Exp Ther Med. 2017 Mar;13(3):924-934
pubmed: 28450921
Cancers (Basel). 2019 Jul 20;11(7):
pubmed: 31330822
J Transl Med. 2011 Dec 07;9:209
pubmed: 22152097
J Clin Pathol. 2007 Oct;60(10):1112-6
pubmed: 17182662
Onco Targets Ther. 2015 Dec 16;8:3783-92
pubmed: 26719706
Carbohydr Polym. 2018 Sep 1;195:243-256
pubmed: 29804974
Asian Pac J Cancer Prev. 2015;16(9):3715-21
pubmed: 25987027
J Cell Physiol. 2007 Jul;212(1):42-50
pubmed: 17311291
Foods. 2016 Nov 29;5(4):
pubmed: 28231175
Curr Opin Plant Biol. 2004 Dec;7(6):661-9
pubmed: 15491914
EMBO J. 2011 Jun 24;30(15):3186-99
pubmed: 21701559
Int J Biol Macromol. 2019 Feb 1;122:64-71
pubmed: 30342944
Nature. 2015 May 14;521(7551):S1
pubmed: 25970450
J Biol Chem. 2002 Nov 29;277(48):45715-8
pubmed: 12364342
Mol Cancer. 2010 Dec 02;9:310
pubmed: 21126356
Int J Cancer. 2010 Aug 15;127(4):873-80
pubmed: 20013803
Planta Med. 2014 Aug;80(13):1107-12
pubmed: 25127022
Carbohydr Res. 2009 Sep 28;344(14):1879-900
pubmed: 19616198
Gastroenterology. 2000 Nov;119(5):1209-18
pubmed: 11054378
Carbohydr Res. 2016 Apr 7;424:30-41
pubmed: 26974354
Cold Spring Harb Perspect Biol. 2013 Apr 01;5(4):a008656
pubmed: 23545416
Life Sci. 1997;60(25):2349-59
pubmed: 9194691
Biochem Soc Trans. 2014 Feb;42(1):76-81
pubmed: 24450631
J Biotechnol. 2009 Aug 20;143(2):157-68
pubmed: 19500620
Toxicol Appl Pharmacol. 2018 Jan 15;339:10-23
pubmed: 29174429
Oncotarget. 2014 Jun 15;5(11):3590-606
pubmed: 24946763
Med Oncol. 2011 Mar;28(1):175-81
pubmed: 20165990
PLoS One. 2014 Jan 27;9(1):e87323
pubmed: 24475272
Anticancer Res. 2018 Mar;38(3):1327-1333
pubmed: 29491056
Int J Mol Sci. 2017 Jan 19;18(1):
pubmed: 28106826
Int J Biol Macromol. 2016 Jul;88:424-32
pubmed: 27064087
Int Immunopharmacol. 2011 Sep;11(9):1226-33
pubmed: 21497206
Int J Mol Sci. 2018 Feb 02;19(2):
pubmed: 29393886
Food Nutr Res. 2017 Nov 13;61(1):1399770
pubmed: 31139040
Cancer. 2009 Nov 1;115(21):4946-58
pubmed: 19658179
Int J Biol Macromol. 2016 Oct;91:1199-205
pubmed: 27370747
Biochemistry (Mosc). 2003 Aug;68(8):882-9
pubmed: 12948389
Am J Physiol Gastrointest Liver Physiol. 2016 Jun 1;310(11):G1081-90
pubmed: 27102559
Carbohydr Polym. 2019 Apr 15;210:412-428
pubmed: 30732778
Food Chem Toxicol. 2006 Oct;44(10):1724-9
pubmed: 16842898
Nature. 2012 Oct 25;490(7421):462-5
pubmed: 23099381
J Polym Environ. 2021;29(8):2359-2371
pubmed: 33526994
Appl Microbiol Biotechnol. 2018 Oct;102(20):8661-8675
pubmed: 30099571
Front Pharmacol. 2017 Mar 03;8:102
pubmed: 28316571
J Agric Food Chem. 2014 Feb 19;62(7):1581-9
pubmed: 24506418
Microbiol Mol Biol Rev. 2013 Sep;77(3):380-439
pubmed: 24006470
Nature. 1999 Apr 1;398(6726):422-6
pubmed: 10201372
Cancers (Basel). 2019 Feb 23;11(2):
pubmed: 30813432
PLoS One. 2015 Mar 24;10(3):e0121674
pubmed: 25803711
Cancer Cell Int. 2016 May 26;16:39
pubmed: 27231438
BMC Cancer. 2010 Sep 03;10:477
pubmed: 20815902
Int J Mol Sci. 2014 May 22;15(5):9103-16
pubmed: 24857920
Biochemistry (Mosc). 2013 Jul;78(7):836-53
pubmed: 24010845
Int J Biol Macromol. 2020 Apr 1;148:843-850
pubmed: 31982521
Oxid Med Cell Longev. 2016;2016:5692852
pubmed: 26682009
Br J Cancer. 2003 Jul 7;89(1):192-8
pubmed: 12838323
Biochim Biophys Acta. 2009 Apr;1795(2):117-29
pubmed: 19167459
Oncol Rep. 2009 Feb;21(2):351-5
pubmed: 19148507
Cell. 2000 Jan 7;100(1):57-70
pubmed: 10647931
Br J Cancer. 1972 Aug;26(4):239-57
pubmed: 4561027
Carbohydr Polym. 2012 Oct 15;90(3):1210-5
pubmed: 22939333
Crit Rev Food Sci Nutr. 2007;47(6):599-610
pubmed: 17653983
Evid Based Complement Alternat Med. 2011;2011:358717
pubmed: 19696196
Invest New Drugs. 2004 Nov;22(4):379-89
pubmed: 15292708
Mol Med Rep. 2015 Nov;12(5):7629-36
pubmed: 26397202
Nat Struct Biol. 2002 Jun;9(6):436-41
pubmed: 11967569
Environ Health. 2015 Feb 11;14:13
pubmed: 25971433
J Recept Signal Transduct Res. 2015;35(6):600-4
pubmed: 26096166
Oncol Lett. 2015 Feb;9(2):972-978
pubmed: 25624917
Cell Death Differ. 2006 Sep;13(9):1423-33
pubmed: 16676004
Evid Based Complement Alternat Med. 2015;2015:560404
pubmed: 25810744
Physiol Mol Biol Plants. 2012 Apr;18(2):177-83
pubmed: 23573055
Int J Biol Macromol. 2011 Jan 1;48(1):160-4
pubmed: 21087621
Phytother Res. 2012 Jul;26(7):1075-81
pubmed: 22213212
Carbohydr Polym. 2014 Jan 30;101:871-7
pubmed: 24299850
ISRN Oncol. 2012;2012:251632
pubmed: 22701186
Int J Biol Macromol. 2016 Oct;91:23-30
pubmed: 27234494
World J Gastrointest Oncol. 2016 Aug 15;8(8):583-91
pubmed: 27574550
J Exp Bot. 2011 Jan;62(1):371-81
pubmed: 20855456
Nat Rev Mol Cell Biol. 2008 Mar;9(3):231-41
pubmed: 18073771
Science. 1998 Sep 4;281(5382):1509-12
pubmed: 9727977
Food Funct. 2018 Jan 24;9(1):502-510
pubmed: 29243753
Int Immunopharmacol. 2006 Mar;6(3):317-33
pubmed: 16428067