The Chimeric Adenovirus (Ad5/35) Expressing Engineered Spike Protein Confers Immunity against SARS-CoV-2 in Mice and Non-Human Primates.
COVID-19 vaccine
GS linker
SARS-CoV-2
Th1 immune responses
chimeric adenovirus-vectored vaccine
neutralizing activity
variants
Journal
Vaccines
ISSN: 2076-393X
Titre abrégé: Vaccines (Basel)
Pays: Switzerland
ID NLM: 101629355
Informations de publication
Date de publication:
30 Apr 2022
30 Apr 2022
Historique:
received:
06
04
2022
revised:
25
04
2022
accepted:
27
04
2022
entrez:
28
5
2022
pubmed:
29
5
2022
medline:
29
5
2022
Statut:
epublish
Résumé
Several COVID-19 platforms have been licensed across the world thus far, but vaccine platform research that can lead to effective antigen delivery is still ongoing. Here, we constructed AdCLD-CoV19 that could modulate humoral immunity by harboring SARS-CoV-2 antigens onto a chimeric adenovirus 5/35 platform that was effective in cellular immunity. By replacing the S1/S2 furin cleavage sequence of the SARS-CoV-2 Spike (S) protein mounted on AdCLD-CoV19 with the linker sequence, high antigen expression was confirmed in various cell lines. The high levels of antigen expression contributed to antigen-specific antibody activity in mice and non-human primates (NHPs) with a single vaccination of AdCLD-CoV19. Furthermore, the adenovirus-induced T
Identifiants
pubmed: 35632468
pii: vaccines10050712
doi: 10.3390/vaccines10050712
pmc: PMC9147121
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Research Foundation of Korea
ID : 2020M3A9I2107463
Organisme : ministry of science and ICT of korea
ID : PRM1752011
Organisme : Korea Research Institute of Bioscience and Biotechnology
ID : KGM4572121
Références
JAMA. 2020 Apr 7;323(13):1239-1242
pubmed: 32091533
Chem Sci. 2019 Oct 7;10(44):10428-10435
pubmed: 32110335
N Engl J Med. 2020 Nov 12;383(20):1920-1931
pubmed: 32663912
Gene Ther. 2004 Oct;11 Suppl 1:S10-7
pubmed: 15454952
Science. 2021 Dec 10;374(6573):1353-1360
pubmed: 34698504
BMJ. 2021 Feb 5;372:n359
pubmed: 33547053
ACS Cent Sci. 2021 Apr 28;7(4):594-602
pubmed: 34056089
J Clin Med. 2020 Feb 20;9(2):
pubmed: 32093211
Nat Microbiol. 2021 Jul;6(7):821-823
pubmed: 34108654
Gene Ther. 2014 Jan;21(1):106-14
pubmed: 24225639
Blood. 2007 Sep 15;110(6):1916-23
pubmed: 17510320
Mol Biosyst. 2017 Feb 28;13(3):598-606
pubmed: 28181620
J Chem Inf Model. 2022 Jan 24;62(2):412-422
pubmed: 34989238
FEBS Lett. 2019 Dec;593(24):3461-3483
pubmed: 31769012
Lancet. 2020 Aug 15;396(10249):467-478
pubmed: 32702298
Eur Respir J. 2020 May 27;55(5):
pubmed: 32312864
Nat Rev Microbiol. 2021 Jul;19(7):409-424
pubmed: 34075212
Cell. 2020 Oct 1;183(1):158-168.e14
pubmed: 32979941
Acta Pharmacol Sin. 2020 Sep;41(9):1141-1149
pubmed: 32747721
Nature. 2020 Aug;584(7821):353-363
pubmed: 32659783
Mol Ther. 2006 Apr;13(4):756-65
pubmed: 16461009
JAMA. 2021 Apr 20;325(15):1535-1544
pubmed: 33704352
Cell. 2020 Aug 20;182(4):812-827.e19
pubmed: 32697968
Nature. 2021 Feb;590(7844):140-145
pubmed: 33137809
Adv Drug Deliv Rev. 2013 Oct;65(10):1357-69
pubmed: 23026637
Nat Rev Immunol. 2020 Oct;20(10):615-632
pubmed: 32887954
Nature. 2021 Aug;596(7871):276-280
pubmed: 34237773
Cell. 2019 Apr 18;177(3):524-540
pubmed: 31002794
Nat Med. 2021 Jul;27(7):1205-1211
pubmed: 34002089
N Engl J Med. 2020 Dec 17;383(25):2427-2438
pubmed: 32991794
Mol Ther. 2020 Mar 4;28(3):709-722
pubmed: 31968213
Nature. 2020 Oct;586(7830):583-588
pubmed: 32731257
Nat Rev Microbiol. 2021 Mar;19(3):141-154
pubmed: 33024307
Nat Commun. 2020 May 20;11(1):2601
pubmed: 32433465
Nat Rev Microbiol. 2019 Mar;17(3):181-192
pubmed: 30531947
Nat Commun. 2020 Aug 14;11(1):4081
pubmed: 32796842
NPJ Vaccines. 2020 Sep 28;5:91
pubmed: 33083026
Science. 2020 May 29;368(6494):945-946
pubmed: 32385100
Cell. 2020 Sep 3;182(5):1284-1294.e9
pubmed: 32730807
N Engl J Med. 2021 Aug 12;385(7):585-594
pubmed: 34289274
Int J Infect Dis. 2020 Feb;91:264-266
pubmed: 31953166
Signal Transduct Target Ther. 2020 Oct 13;5(1):237
pubmed: 33051445
Lancet Infect Dis. 2021 Aug;21(8):1070
pubmed: 34022142
Nat Rev Immunol. 2020 Jun;20(6):363-374
pubmed: 32346093
Front Immunol. 2020 Sep 28;11:581807
pubmed: 33117391
J Infect Dis. 2020 Oct 13;222(10):1596-1600
pubmed: 32745172
Clin Chem. 2020 Apr 1;66(4):549-555
pubmed: 32031583
Signal Transduct Target Ther. 2020 Jun 12;5(1):92
pubmed: 32532959
N Engl J Med. 2020 Dec 17;383(25):2439-2450
pubmed: 33053279