Cancer
Cell Biology
Gene Expression
Metabolism
Microscopy
Model Organisms
Molecular Biology
Molecular/Chemical Probes
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 06 2022
17 06 2022
Historique:
entrez:
31
5
2022
pubmed:
1
6
2022
medline:
3
6
2022
Statut:
epublish
Résumé
We provide a protocol for gain-of-function (GOF) cDNA screen of genes that foster cancer cell colonization of secondary tissues, the last and most lethal step of the metastasis cascade. We present techniques for cDNA viral library preparation and delivery leading up to the recovery of colonization-promoting sequences in a proof-of-concept DU145-based mouse model of pulmonary metastasis. Adapted to other cDNA libraries and cancer models, this approach would prove widely useful in enumerating intrinsic genetic determinants underlying metastatic colonization. For complete details on the use and execution of this protocol, please refer to Tu et al. (2021).
Identifiants
pubmed: 35634360
doi: 10.1016/j.xpro.2022.101413
pii: S2666-1667(22)00293-3
pmc: PMC9136352
doi:
Substances chimiques
DNA, Complementary
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101413Subventions
Organisme : NCI NIH HHS
ID : R01 CA207322
Pays : United States
Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
RNA. 2003 Apr;9(4):493-501
pubmed: 12649500
iScience. 2021 Apr 20;24(5):102425
pubmed: 34036247