Retinol dehydrogenase 10 contributes to cancer stemness and intracellular carbohydrate storage in ovarian clear cell carcinomas.

Adenocarcinoma, Clear Cell / metabolism Alcohol Oxidoreductases / metabolism Carbohydrate Metabolism Female Humans Neoplastic Stem Cells Ovarian Neoplasms / metabolism
Ovarian clear cell carcinoma RDH10 carbohydrates gluconeogenesis retinoids

Journal

Cancer biomarkers : section A of Disease markers
ISSN: 1875-8592
Titre abrégé: Cancer Biomark
Pays: Netherlands
ID NLM: 101256509

Informations de publication

Date de publication:
2022
Historique:
pubmed: 1 6 2022
medline: 3 8 2022
entrez: 31 5 2022
Statut: ppublish

Résumé

Ovarian clear cell carcinomas (OCCCs) have been recurrent and refractory among the present treatments, so novel therapeutics are urgently needed. The present study accumulates the proof of concept to examine the feasibility of RDH10 as a therapeutic target for treating OCCCs. Immunohistochemically, RDH10 expression was evaluated in 111 primary epithelial ovarian cancers, including 55 OCCCs, 31 ovarian endometrioid carcinomas and 25 ovarian serous carcinomas. The spherogenecity provoked by RDH10 was evaluated in OCCC cells. To analyze whether RDH10 promotes carbohydrate storage via the vitamin A-gluconeogenesis pathway, phosphoenolpyruvate carboxykinase 1 (PCK1) protein levels and intracellular carbohydrate content were measured in response to modified RDH10 expression. Abundant RDH10 was expressed specifically in OCCCs. RDH10 promoted spherogenecity and intracellular carbohydrate storage via modulation of PCK1 expression in OCCC cells. In the present study, abundant RDH10 contributed to cancer cell stemness and intracellular carbohydrate storage in OCCCs. RDH10 is a potentially, new therapeutic candidate for treating OCCC cases.

Sections du résumé

BACKGROUND BACKGROUND
Ovarian clear cell carcinomas (OCCCs) have been recurrent and refractory among the present treatments, so novel therapeutics are urgently needed.
OBJECTIVE OBJECTIVE
The present study accumulates the proof of concept to examine the feasibility of RDH10 as a therapeutic target for treating OCCCs.
METHODS METHODS
Immunohistochemically, RDH10 expression was evaluated in 111 primary epithelial ovarian cancers, including 55 OCCCs, 31 ovarian endometrioid carcinomas and 25 ovarian serous carcinomas. The spherogenecity provoked by RDH10 was evaluated in OCCC cells. To analyze whether RDH10 promotes carbohydrate storage via the vitamin A-gluconeogenesis pathway, phosphoenolpyruvate carboxykinase 1 (PCK1) protein levels and intracellular carbohydrate content were measured in response to modified RDH10 expression.
RESULTS RESULTS
Abundant RDH10 was expressed specifically in OCCCs. RDH10 promoted spherogenecity and intracellular carbohydrate storage via modulation of PCK1 expression in OCCC cells.
CONCLUSIONS CONCLUSIONS
In the present study, abundant RDH10 contributed to cancer cell stemness and intracellular carbohydrate storage in OCCCs. RDH10 is a potentially, new therapeutic candidate for treating OCCC cases.

Identifiants

DOI: 10.3233/CBM-210435 PMID: 35634847
pubmed: 35634847
pii: CBM210435
doi: 10.3233/CBM-210435
doi:

Substances chimiques

Alcohol Oxidoreductases EC 1.1.-
trans-retinol dehydrogenase EC 1.1.1.-
retinol dehydrogenase EC 1.1.1.105

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

673-679

Auteurs

Atsushi Murakami (A)

Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu, Shiga, Japan.

Tsukuru Amano (T)

Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu, Shiga, Japan.

Fumi Yoshino (F)

Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu, Shiga, Japan.

Hiroko Kita (H)

Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Otsu, Shiga, Japan.

Suzuko Moritani (S)

Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Otsu, Shiga, Japan.

Takashi Murakami (T)

Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu, Shiga, Japan.

Tokuhiro Chano (T)

Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Otsu, Shiga, Japan.
Department of Medical Genetics, Shiga University of Medical Science, Otsu, Shiga, Japan.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Tumeurs › Tumeurs par type histologique › Tumeurs épithéliales épidermoïdes et glandulaires › Carcinomes › Adénocarcinome › Adénocarcinome à cellules claires › Retinol dehydrogenase 10 contributes to cancer...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Alcohol oxidoreductases › Retinol dehydrogenase 10 contributes to cancer...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Retinol dehydrogenase 10 contributes to cancer...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Retinol dehydrogenase 10 contributes to cancer...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches tumorales › Retinol dehydrogenase 10 contributes to cancer...
questionsmedicales.fr › Maladies endocriniennes › Troubles gonadiques › Maladies ovariennes › Tumeurs de l'ovaire › Retinol dehydrogenase 10 contributes to cancer...