Immunogenicity and cross-protective efficacy of recombinant H5HA1 protein of clade 2.3.2.1a highly pathogenic H5N1 avian influenza virus expressed in E.coli.
Characterization
Cross-protection
HA1
HPAIV H5N1
Humoral response
Prokaryotic expression
Journal
Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
received:
01
03
2022
revised:
19
05
2022
accepted:
20
05
2022
pubmed:
1
6
2022
medline:
18
6
2022
entrez:
31
5
2022
Statut:
ppublish
Résumé
The global spread of H5N1 highly pathogenic avian influenza virus (HPAIV) in poultry has caused great economic loss to the poultry farmers and industry with significant pandemic threat. The current study involved production of recombinant HA1 protein of clade 2.3.2.1a H5N1 HPAIV (rH5HA1) in E.coli and evaluation of its protective efficacy in chickens. Purification under denaturing conditions and refolding by dialysis against buffers containing decreasing concentrations of urea was found to preserve the biological activity of the expressed recombinant protein as assessed by hemagglutination assay, Western blot and ELISA. The Montanide ISA 71 VGA adjuvanted rH5HA1 protein was used for immunization of chickens. Humoral response was maintained at a minimum of 4log
Identifiants
pubmed: 35636692
pii: S0882-4010(22)00218-2
doi: 10.1016/j.micpath.2022.105605
pii:
doi:
Substances chimiques
Influenza Vaccines
0
Monatide (IMS 3015)
0
Recombinant Proteins
0
Mineral Oil
8020-83-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105605Informations de copyright
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