Visualising the endothelial glycocalyx in dogs.
Alcian blue
Canine
Microvasculature
Transmission electron microscopy
Journal
Veterinary journal (London, England : 1997)
ISSN: 1532-2971
Titre abrégé: Vet J
Pays: England
ID NLM: 9706281
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
received:
14
09
2021
revised:
21
04
2022
accepted:
25
05
2022
pubmed:
1
6
2022
medline:
10
8
2022
entrez:
31
5
2022
Statut:
ppublish
Résumé
The endothelial glycocalyx (eGlx) lines the luminal surface of endothelial cells. It is critical in maintaining vascular health and when damaged contributes to many diseases. Its fragility makes studying the eGlx technically challenging. The current reference standard for eGlx visualisation, by electron microscopy using glutaraldehyde/Alcian blue perfusion fixation, has not been previously reported in dogs. Established techniques were applied to achieve visualisation of the eGlx in the microvasculature of reproductive tissue in five healthy dogs undergoing elective neutering. Uterine and testicular artery samples underwent perfusion fixation, in the presence of Alcian blue, prior to transmission electron microscopy imaging. Image processing software was used to determine eGlx depth. EGlx was visualised in the arteries of two dogs, one testicular and one uterine, with median (range) eGlx depths of 68.2 nm (32.1-122.9 nm) and 47.6 nm (26.1-129.4 nm) respectively. Study of the eGlx is technically challenging, particularly its direct visualisation in clinical samples. Further research is needed to develop more clinically applicable techniques to measure eGlx health.
Identifiants
pubmed: 35640795
pii: S1090-0233(22)00059-4
doi: 10.1016/j.tvjl.2022.105844
pmc: PMC9587350
pii:
doi:
Substances chimiques
Alcian Blue
P4448TJR7J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105844Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.
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