Surface-enhanced Raman spectral investigation of antibacterial activity of zinc 3-chlorobenzoic acid complexes against Gram-positive and Gram-negative bacteria.
Bacillus subtilis
Escherichia coli
Ligand & complex exposure
Principal component analysis
Surface-enhanced Raman spectroscopy
Journal
Photodiagnosis and photodynamic therapy
ISSN: 1873-1597
Titre abrégé: Photodiagnosis Photodyn Ther
Pays: Netherlands
ID NLM: 101226123
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
24
01
2022
revised:
18
05
2022
accepted:
27
05
2022
pubmed:
1
6
2022
medline:
9
9
2022
entrez:
31
5
2022
Statut:
ppublish
Résumé
Raman spectroscopy is an effective tool for determining the antibacterial actitivites of organometallic compounds against different bacterial strains. The purpose of current study is to check the anibacterial activites of ligand (3-chlorobenzoic acid) and its respective zinc complex against gram positive and gram-negative bacterial strains by using surface enhanced Raman spectroscopy (SERS). The ligand (3-chlorobenzoic acid) and its respective zinc complex caused different biochemical changes in gram-positive and gram-negative bacterial strains such as lipid contents, DNA/RNA contents, proteins contents, peptidoglycan contents and bacterial spore contents which can be observed with different SERS spectral features. Furthermore, PCA was employed for differentiating the mechanism of action of zinc complexes against gram-positive bacterial strain and gram-negative bacterial strain. Surface enhanced Raman spectroscopy (SERS) has been employed for analyzing the antibiotic activities of 3-chlorobenzoic acid ligands and their respective zinc complexes against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive) bacterial strains. The bioactivity assay and SERS spectral results clearly show that the complex causes more degradation in both bacterial cells (E. coli and B. subtilis) as compared to ligand. Furthermore, PCA was employed for differentiating the mechanism of action of zinc complexes against gram-positive bacterial strain and gram-negative bacterial strain. SERS technique along with chemometric tools have successfully differentiated the antibiotic activities of 3-chlorobenzoic acid ligands and their respective zinc complexes against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive) bacterial strains.
Sections du résumé
BACKGROUND
BACKGROUND
Raman spectroscopy is an effective tool for determining the antibacterial actitivites of organometallic compounds against different bacterial strains.
OBJECTIVES
OBJECTIVE
The purpose of current study is to check the anibacterial activites of ligand (3-chlorobenzoic acid) and its respective zinc complex against gram positive and gram-negative bacterial strains by using surface enhanced Raman spectroscopy (SERS).
METHODS
METHODS
The ligand (3-chlorobenzoic acid) and its respective zinc complex caused different biochemical changes in gram-positive and gram-negative bacterial strains such as lipid contents, DNA/RNA contents, proteins contents, peptidoglycan contents and bacterial spore contents which can be observed with different SERS spectral features. Furthermore, PCA was employed for differentiating the mechanism of action of zinc complexes against gram-positive bacterial strain and gram-negative bacterial strain.
RESULTS
RESULTS
Surface enhanced Raman spectroscopy (SERS) has been employed for analyzing the antibiotic activities of 3-chlorobenzoic acid ligands and their respective zinc complexes against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive) bacterial strains. The bioactivity assay and SERS spectral results clearly show that the complex causes more degradation in both bacterial cells (E. coli and B. subtilis) as compared to ligand. Furthermore, PCA was employed for differentiating the mechanism of action of zinc complexes against gram-positive bacterial strain and gram-negative bacterial strain.
CONCLUSION
CONCLUSIONS
SERS technique along with chemometric tools have successfully differentiated the antibiotic activities of 3-chlorobenzoic acid ligands and their respective zinc complexes against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive) bacterial strains.
Identifiants
pubmed: 35640835
pii: S1572-1000(22)00227-7
doi: 10.1016/j.pdpdt.2022.102941
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Chlorobenzoates
0
Ligands
0
3-chlorobenzoic acid
02UOJ7064K
Zinc
J41CSQ7QDS
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102941Informations de copyright
Copyright © 2022. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interests The authors have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.