Datura innoxia antimicrobial activities against E. coli isolated from infections of type 2 diabetic patients.

The current study was designed to explore the antimicrobial activity from plant sources in order to inhibit the microorganisms responsible for infections. 137 samples were collected from hospitals of district Attock. Agar disc diffusion was used to check the resistance patterns. Datura innoxia activity was checked against strains. Integron (class 1) presence was detected through PCR based study. Ethanolic and metabolic extracts were effective against E. coli strains. Ethanolic leaf extract showed more activity while chloroform and methanolic extracts were least activities. FTIR analysis of the data showed that plant showed antimicrobial activity due to presence of different compounds including alcoholic, aromatic, carboxlic acid, alkane as main compounds. Results of biofilm detection showed that 69% strains were able to develop strong biofilm. Report of (class 1) integron was really significant because it was reported first time from this area. 16S r DNA also confirmed the results. The Datura innoxia extracts activity against E. coli was also really significant. This will really help to find out the reason behind prolonged stay of diabetic patients in hospitals and plant activity against strains will really help to explore potent herbal agents against pathogenic strains.