GMAP is an Atg8a-interacting protein that regulates Golgi turnover in Drosophila.
CP: Cell biology
CP: Immunology
Golgi
Golgiphagy Drosophila
LIR motif
LIR motif docking site
autophagy
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
31 05 2022
31 05 2022
Historique:
received:
02
09
2021
revised:
11
03
2022
accepted:
11
05
2022
entrez:
1
6
2022
pubmed:
2
6
2022
medline:
7
6
2022
Statut:
ppublish
Résumé
Selective autophagy receptors and adapters contain short linear motifs called LIR motifs (LC3-interacting region), which are required for the interaction with the Atg8-family proteins. LIR motifs bind to the hydrophobic pockets of the LIR motif docking site (LDS) of the respective Atg8-family proteins. The physiological significance of LDS docking sites has not been clarified in vivo. Here, we show that Atg8a-LDS mutant Drosophila flies accumulate autophagy substrates and have reduced lifespan. Using quantitative proteomics to identify the proteins that accumulate in Atg8a-LDS mutants, we identify the cis-Golgi protein GMAP (Golgi microtubule-associated protein) as a LIR motif-containing protein that interacts with Atg8a. GMAP LIR mutant flies exhibit accumulation of Golgi markers and elongated Golgi morphology. Our data suggest that GMAP mediates the turnover of Golgi by selective autophagy to regulate its morphology and size via its LIR motif-mediated interaction with Atg8a.
Identifiants
pubmed: 35649355
pii: S2211-1247(22)00678-7
doi: 10.1016/j.celrep.2022.110903
pmc: PMC9637997
pii:
doi:
Substances chimiques
Autophagy-Related Protein 8 Family
0
Microtubule-Associated Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110903Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L006324/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P007856/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/V014838/1
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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