[Isolating Venomous Constituents of Paralepistopsis acromelalgaand Simultaneous Analysis of Its Compounds].
Acromelic acid
Clitidine
Eluting Position Controllable Smart Flash
Erythromelalgia
LC-MS/MS
Paralepistopsis acromelalga
Journal
Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
ISSN: 0015-6426
Titre abrégé: Shokuhin Eiseigaku Zasshi
Pays: Japan
ID NLM: 0142214
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
1
6
2022
pubmed:
2
6
2022
medline:
7
6
2022
Statut:
ppublish
Résumé
An isolation method for Acromelic acids A, B and Clitidine, which are venomous constituents of Paralepistopsis acromelalga was developed. Highly purified products were obtained from the mushroom extract using silica gel, ODS, ion-exchange column chromatography and preparative TLC. Using those results, we optimized the LC-MS/MS conditions. Finally we developed a method for simultaneous analysis. In recovery tests, the average recovery was 80.8-112.4%, repeatability was 1.4-3.8RSD%. The limits of quantification of the respective compounds were estimated as 0.25 μg/g. Based on the results, this method can reveal causes of food poisoning by Paralepistopsis acromelalga.
Identifiants
pubmed: 35650032
doi: 10.3358/shokueishi.63.79
doi:
Substances chimiques
Venoms
0
Types de publication
Journal Article
Langues
jpn
Sous-ensembles de citation
IM