In vitro generation of transplantable insulin-producing cells from canine adipose-derived mesenchymal stem cells.

Animals Cell Differentiation Dogs Insulin / pharmacology Mesenchymal Stem Cells Niacinamide / pharmacology Phosphatidylinositol 3-Kinases Taurine / pharmacology Transforming Growth Factor beta / pharmacology

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
01 06 2022
Historique:
received: 18 01 2022
accepted: 20 05 2022
entrez: 1 6 2022
pubmed: 2 6 2022
medline: 7 6 2022
Statut: epublish

Résumé

Canine mesenchymal stem cells (cMSCs) have potential applications for regenerative therapy, including the generation of insulin-producing cells (IPCs) for studying and treating diabetes. In this study, we established a useful protocol for generating IPCs from canine adipose mesenchymal stem cells (cAD-MSCs). Subsequently, in vitro preservation of pluronic F127-coated alginate (ALGPA)-encapsulated cAD-MSC-derived IPCs was performed to verify ready-to-use IPCs. IPCs were induced from cAD-MSCs with the modulated three-stepwise protocol. The first step of definitive endoderm (DE) induction showed that the cooperation of Chir99021 and Activin A created the effective production of Sox17-expressed DE cells. The second step for pancreatic endocrine (PE) progenitor induction from DE indicated that the treatment with taurine, retinoic acid, FGF2, EGF, TGFβ inhibitor, dorsomorphin, nicotinamide, and DAPT showed the significant upregulation of the pancreatic endocrine precursor markers Pdx1 and Ngn3. The last step of IPC production, the combination of taurine, nicotinamide, Glp-1, forskolin, PI3K inhibitor, and TGFβ inhibitor, yielded efficiently functional IPCs from PE precursors. Afterward, the maintenance of ALGPA-encapsulated cAD-MSC-derived IPCs with VSCBIC-1, a specialized medium, enhanced IPC properties. Conclusion, the modulated three-stepwise protocol generates the functional IPCs. Together, the encapsulation of cAD-MSC-derived IPCs and the cultivation with VSCBIC-1 enrich the maturation of generated IPCs.

Identifiants

DOI: 10.1038/s41598-022-13114-3 PMID: 35650303 PMC: PMC9160001
pubmed: 35650303
doi: 10.1038/s41598-022-13114-3
pii: 10.1038/s41598-022-13114-3
pmc: PMC9160001
doi:

Substances chimiques

Insulin 0
Transforming Growth Factor beta 0
Taurine 1EQV5MLY3D
Niacinamide 25X51I8RD4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

9127

Informations de copyright

© 2022. The Author(s).

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Auteurs

Quynh Dang Le (Q)

International Program of Veterinary Science and Technology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

Watchareewan Rodprasert (W)

Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

Suryo Kuncorojakti (S)

Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.

Prasit Pavasant (P)

Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Center of Excellence in Regenerative Dentistry (CERD), Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.

Thanaphum Osathanon (T)

Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.

Chenphop Sawangmake (C)

Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. chenphop.s@chula.ac.th.
Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. chenphop.s@chula.ac.th.
Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. chenphop.s@chula.ac.th.
Center of Excellence in Regenerative Dentistry (CERD), Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand. chenphop.s@chula.ac.th.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › In vitro generation of transplantable...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › In vitro generation of transplantable...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Carnivora › Pinnipedia › Canidae › Chiens › In vitro generation of transplantable...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Hormones peptidiques › Hormones pancréatiques › Insulines › Insuline › In vitro generation of transplantable...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › In vitro generation of transplantable...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyridines › Acides nicotiniques › Nicotinamide › In vitro generation of transplantable...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Phosphatidylinositol 3-kinases › In vitro generation of transplantable...
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Acides du soufre › Acides sulfoniques › Acides alcanesulfoniques › Taurine › In vitro generation of transplantable...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines de la superfamille du TGF bêta › Facteur de croissance transformant bêta › In vitro generation of transplantable...