Adaptation to simulated microgravity in Streptococcus mutans.
Journal
NPJ microgravity
ISSN: 2373-8065
Titre abrégé: NPJ Microgravity
Pays: United States
ID NLM: 101703605
Informations de publication
Date de publication:
02 Jun 2022
02 Jun 2022
Historique:
received:
03
09
2021
accepted:
13
05
2022
entrez:
2
6
2022
pubmed:
3
6
2022
medline:
3
6
2022
Statut:
epublish
Résumé
Long-term space missions have shown an increased incidence of oral disease in astronauts' and as a result, are one of the top conditions predicted to impact future missions. Here we set out to evaluate the adaptive response of Streptococcus mutans (etiological agent of dental caries) to simulated microgravity. This organism has been well studied on earth and treatment strategies are more predictable. Despite this, we are unsure how the bacterium will respond to the environmental stressors in space. We used experimental evolution for 100-days in high aspect ratio vessels followed by whole genome resequencing to evaluate this adaptive response. Our data shows that planktonic S. mutans did evolve variants in three genes (pknB, SMU_399 and SMU_1307c) that can be uniquely attributed to simulated microgravity populations. In addition, collection of data at multiple time points showed mutations in three additional genes (SMU_399, ptsH and rex) that were detected earlier in simulated microgravity populations than in the normal gravity controls, many of which are consistent with other studies. Comparison of virulence-related phenotypes between biological replicates from simulated microgravity and control orientation cultures generally showed few changes in antibiotic susceptibility, while acid tolerance and adhesion varied significantly between biological replicates and decreased as compared to the ancestral populations. Most importantly, our data shows the importance of a parallel normal gravity control, sequencing at multiple time points and the use of biological replicates for appropriate analysis of adaptation in simulated microgravity.
Identifiants
pubmed: 35654802
doi: 10.1038/s41526-022-00205-8
pii: 10.1038/s41526-022-00205-8
pmc: PMC9163064
doi:
Types de publication
Journal Article
Langues
eng
Pagination
17Subventions
Organisme : NIGMS NIH HHS
ID : T34 GM083980
Pays : United States
Organisme : National Science Foundation (NSF)
ID : DBI-0939454
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : 5T34GM083980
Informations de copyright
© 2022. The Author(s).
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